Effects of endocrine disruptors on genes associated with 17beta-estradiol metabolism and excretion

• Published in: Steroids Vol. 73; no. 12; p. 1242
• Main Authors: Hanet, Nathalie, Lancon, Allan, Delmas, Dominique, Jannin, Brigitte, Chagnon, Marie-Christine, Cherkaoui-Malki, Moustapha, Latruffe, Norbert, Artur, Yves, Heydel, Jean-Marie
• Format: Journal Article
• Language: English
• Published: United States 01.11.2008
• Subjects: Base Sequence; Blotting, Western; Cell Line; DNA Primers; Endocrine Disruptors - pharmacology; Estradiol - metabolism; Gene Expression Regulation - drug effects; Humans; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics

In order to provide a global analysis of the effects of endocrine disruptors on the hormone cellular bioavailability, we combined 17beta-estradiol (E2) cellular flow studies with real-time PCR and Western blot expression measurements of genes involved in the hormone metabolism and excretion. Three e...