Ceramide generation in situ alters leukocyte cytoskeletal organization and beta 2-integrin function and causes complete degranulation

• Published in: The Journal of biological chemistry Vol. 277; no. 6; p. 4285
• Main Authors: Feldhaus, Michael J, Weyrich, Andrew S, Zimmerman, Guy A, McIntyre, Thomas M
• Format: Journal Article
• Language: English
• Published: United States 08.02.2002
• Subjects: Actins - metabolism; CD18 Antigens - metabolism; Cell Degranulation; Cells, Cultured; Ceramides - biosynthesis; Cytochalasin D - pharmacology; Cytoskeleton - ultrastructure; Humans; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophils - drug effects; Neutrophils - enzymology; Neutrophils - metabolism; Neutrophils - ultrastructure; Sphingomyelin Phosphodiesterase - metabolism; Superoxides - metabolism

Ceramide levels increase in activated polymorphonuclear neutrophils, and here we show that endogenous ceramide induced degranulation and superoxide generation and increased surface beta(2)-integrin expression. Ceramide accumulation reveals a bifurcation in integrin function, as it abolished agonist-...