Hemopoiesis on stromal sublayers formed by constant lines of fibroblasts

We studied the formation of hemopoietic colonies on an artificial sublayer in the peritoneal cavity of mice under the influence of stromal sublayers consisting of fibroblasts of six constant cell limes. All studied lines of stromal cells supported the formation of granulocytic foci and some of them...

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Bibliographic Details
Published inIzvestiia Akademii nauk. Seriia biologicheskaia no. 4; p. 393
Main Authors Michurina, T V, Paiushina, O V, Bueverova, E I, Satdykova, G P, Bragina, E V, Nikonova, T M, Khrushchov, N G
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.2002
Subjects
3T3 Cells
Animals
Blood Cells - cytology
Blood Cells - drug effects
Cell Line
Cell Transplantation
Cytokines - metabolism
Erythrocytes - drug effects
Escherichia coli
Female
Fibroblasts - drug effects
Fibroblasts - metabolism
Glutaral - pharmacology
Granulocytes - drug effects
Hematopoiesis - physiology
Humans
Lipopolysaccharides - pharmacology
Mice
Mice, Inbred Strains
Peritoneal Cavity - cytology
Rats
Stromal Cells
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Summary:We studied the formation of hemopoietic colonies on an artificial sublayer in the peritoneal cavity of mice under the influence of stromal sublayers consisting of fibroblasts of six constant cell limes. All studied lines of stromal cells supported the formation of granulocytic foci and some of them supported the formation of erythroid foci as well. It was shown that hemopoiesis was preserved or, in some cases, enhanced on sublayers of fixed (metabolically inactive) cells. The treatment of fibroblasts by E. coli lipopolysaccharide did not lead, as a rule, to significant stimulation of hemopoiesis.
ISSN:1026-3470