Quantitative assessment of the processes of insulin synthesis and secretion in B-endocrinocytes of pancreatic islets

• Published in: Morfologiia (Saint Petersburg, Russia) Vol. 122; no. 5; p. 70
• Main Authors: Kolesnik, Iu M, Orlovskiĭ, M A, Abramov, A V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2002
• Subjects: Animals; Blood Glucose - analysis; Fluorescent Antibody Technique, Indirect; Glucose - pharmacology; Injections, Intraperitoneal; Insulin - biosynthesis; Insulin - blood; Insulin - metabolism; Insulin Secretion; Islets of Langerhans - cytology; Islets of Langerhans - drug effects; Islets of Langerhans - metabolism; Male; Microscopy, Fluorescence; Rats; Rats, Wistar

Immunofluorescent methods allowing the detection of different hormones in endocrine cells, are widely used in many works, however, their application is usually limited to the description of qualitative criteria, while the reactions of indirect immunofluorescence permit their quantitative assessment....