Identification of a new pebp2alphaA2 isoform from zebrafish runx2 capable of inducing osteocalcin gene expression in vitro

• Published in: Journal of bone and mineral research Vol. 20; no. 8; p. 1440
• Main Authors: Pinto, Jorge P, Conceição, Natércia M, Viegas, Carla S B, Leite, Ricardo B, Hurst, Laurence D, Kelsh, Robert N, Cancela, M Leonor
• Format: Journal Article
• Language: English
• Published: United States 01.08.2005
• Subjects: Amino Acid Sequence; Animals; Conserved Sequence; Evolution, Molecular; Gene Expression Regulation; Genes, Reporter - genetics; Molecular Sequence Data; Osteocalcin - genetics; Phylogeny; Promoter Regions, Genetic; Protein Conformation; Protein Isoforms - classification; Protein Isoforms - genetics; Protein Isoforms - metabolism; Transcription Factors - classification; Transcription Factors - genetics; Transcription Factors - metabolism; Up-Regulation; Zebrafish - genetics; Zebrafish - metabolism; Zebrafish Proteins - classification; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism

The zebrafish runx2b transcription factor is an ortholog of RUNX2 and is highly conserved at the structural level. The runx2b pebp2alphaA2 isoform induces osteocalcin gene expression by binding to a specific region of the promoter and seems to have been selectively conserved in the teleost lineage....