Phosphoinositide-3-kinase-independent contractile activities associated with Fcgamma-receptor-mediated phagocytosis and macropinocytosis in macrophages

• Published in: Journal of cell science Vol. 116; no. Pt 2; pp. 247 - 257
• Main Authors: Araki, Nobukazu, Hatae, Tanenori, Furukawa, Aizo, Swanson, Joel A
• Format: Journal Article
• Language: English
• Published: England 15.01.2003
• Subjects: Actin Cytoskeleton - drug effects; Actin Cytoskeleton - genetics; Actin Cytoskeleton - metabolism; Actin Cytoskeleton - ultrastructure; Androstadienes - pharmacology; Animals; Azepines - pharmacology; Cell Surface Extensions - drug effects; Cell Surface Extensions - metabolism; Cell Surface Extensions - ultrastructure; Cells, Cultured; Contractile Proteins - drug effects; Contractile Proteins - metabolism; Enzyme Inhibitors - pharmacology; Erythrocytes - cytology; Erythrocytes - metabolism; Macrophages - drug effects; Macrophages - enzymology; Macrophages - ultrastructure; Mice; Microscopy, Electron, Scanning; Myosin-Light-Chain Kinase - antagonists & inhibitors; Myosin-Light-Chain Kinase - metabolism; Myosins - metabolism; Naphthalenes - pharmacology; Phagocytosis - drug effects; Phagocytosis - physiology; Phosphatidylinositol 3-Kinases - antagonists & inhibitors; Phosphatidylinositol 3-Kinases - metabolism; Pinocytosis - drug effects; Pinocytosis - physiology; Receptors, IgG - drug effects; Receptors, IgG - metabolism; Recombinant Fusion Proteins; Wortmannin

Previous studies have shown that Fcgamma receptor (FcR)-mediated phagocytosis and macropinocytosis in macrophages consist of two dissociable activities: a phosphoinositide 3-kinase (PI3K)-independent extension of phagocytic cups and a PI3K-dependent contractile mechanism that closes phagosomes and r...