Structure of interphase chromatin of diploid and trisomal human cells studied by the technic of thermal denaturation in media of low ionic strength

• Published in: Biulleten' eksperimental'noi biologii i meditsiny Vol. 92; no. 12; p. 717
• Main Authors: Fedorova, K N, Shchepilov, V N, Iudina, I E
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.12.1981
• Subjects: Chromatin; Deoxyribonucleoproteins; DNA; Down Syndrome - genetics; Humans; Lymphocytes; Microscopy, Fluorescence; Nucleic Acid Denaturation; Osmolar Concentration; Protein Denaturation; Temperature

Fluorescent microscopy with the use of acridine orange has shown on a short-term human cell culture that the melting profiles of intact lymphocytes from normal subjects present the curves with maxima (F530) within a definite temperature range: 45, 65, 78, 85, 88 and 92 degrees C (+/- degrees C). The...