Characterization and differentiation of rat preadipocytes in primary culture

Using a density gradient medium, we isolated homogeneous cell populations from the inguinal tissue of 3-day old rats. In primary culture we obtained, for the first time, the differentiation of 99% of the cells in the presence of a physiological concentration of insulin (10(-9) M). This model closely...

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Published inReproduction, nutrition, développement Vol. 25; no. 1B; pp. 165 - 167
Main Authors Gaben-Cogneville, A M, Quignard-Boulangé, A, Aron, Y, Brigant, L, Jahchan, T, Pello, J Y, Poussin, B, Swierczewski, E
Format Journal Article
LanguageFrench
Published France 1985
Subjects
Adipose Tissue - cytology
Adipose Tissue - metabolism
Animals
Cell Differentiation - drug effects
Cells, Cultured
Cyclic AMP - biosynthesis
Glucagon - pharmacology
Insulin - pharmacology
Isoproterenol - pharmacology
Lipid Metabolism
Lipoprotein Lipase - metabolism
Lipoproteins, VLDL - pharmacology
Rats
Stem Cells - cytology
Triglycerides - metabolism
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Summary:Using a density gradient medium, we isolated homogeneous cell populations from the inguinal tissue of 3-day old rats. In primary culture we obtained, for the first time, the differentiation of 99% of the cells in the presence of a physiological concentration of insulin (10(-9) M). This model closely mimicked the events occurring during normal mammalian adipose development, i.e. a positive change in beta-adrenergic sensitivity, early induction of lipoprotein lipase, expression of the enzymes involved in the triglyceride systems, and the development of responsiveness to glucagon.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0181-1916