Integration of the genome of the Mu-like Pseudomonas aeruginosa bacteriophage D3112 into plasmid RP4 and its hybrid plasmid transfer into Pseudomonas putida and Escherichia coli C600 bacteria

• Published in: Genetika Vol. 18; no. 1; p. 5
• Main Authors: Krylov, V N, Plotnikova, T G, Kulakov, L A, Fedorova, T V, Eremenko, E N
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 1982
• Subjects: Bacteriophages - genetics; Conjugation, Genetic; Crosses, Genetic; Escherichia coli - genetics; Genes, Viral; Genetic Markers; Lysogeny; Plasmids; Pseudomonas - genetics; Pseudomonas aeruginosa

The genome of a Mu-like bacteriophage D3112 specific for Pseudomonas aeruginosa was integrated in vivo into the RP4 plasmid. The fact of integration has been proved by two experiments: 1. The loss of RP4 plasmid is accompanied by loss of D3112 prophage; 2. Transfer of the plasmid by conjugation from...