On the fluorimetric determination of oestriol in pregnancy urines after thin layer chromatography

• Published in: Zeitschrift für klinische Chemie und klinische Biochemie Vol. 13; no. 9; p. 381
• Main Authors: van Bezeij, M, Bosch, M W
• Format: Journal Article
• Language: English
• Published: Germany 01.09.1975
• Subjects: Birth Weight; Chromatography, Thin Layer - methods; Dansyl Compounds; Estriol - urine; Female; Glycosuria; Humans; Infant, Newborn; Microchemistry; Pregnancy; Pregnancy Trimester, Third; Probability; Spectrometry, Fluorescence - methods

An improved method is described for the determination of oestriol concentrations in urines of pregnant women. Oestriol is detected as a derivate of 1-dimethylaminonaphtalene-5-sulphonylchloride (DANSYLchloride). The method has the following characteristics: 1. The recovery of oestriol-16-glucuronide...