Optimization of a protocol for automatic solid phase synthesis of peptides using a variable volume flow reactor

• Published in: Bioorganicheskaia khimiia Vol. 21; no. 3; p. 179
• Main Authors: Moshnikov, S A, Mustaeva, L G, Danilov, A V, Sukhov, I E, Baru, M B
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.03.1995
• Subjects: Amino Acid Sequence; Amino Acids - chemistry; Chromatography, Gel; Chromatography, High Pressure Liquid; Equipment and Supplies; Indicators and Reagents; Methods; Molecular Sequence Data; Peptides - chemical synthesis; Peptides - isolation & purification; Solvents

The protocol for solid phase peptide synthesis using automatic synthesizers MilliGen/Biosearch 9500 and 9600 with built-in variable volume flow-reactors has been elaborated. The data of swellographic monitoring were used for optimization of process parameters. The peptides involving from 10 to 21 am...