The structure of nucleosomal core particles located on the transcribed genome regions

• Published in: Molekuliarnaia biologiia Vol. 22; no. 3; p. 706
• Main Authors: Studitskiĭ, V M, Beliavskiĭ, A V, Mel'nikova, A F, Mirzabekov, A D
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.1988
• Subjects: Animals; Drosophila melanogaster - genetics; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Heat-Shock Proteins - genetics; Histones - genetics; Histones - ultrastructure; Nucleosomes - analysis; Nucleosomes - ultrastructure; Transcription, Genetic
• ISSN: 0026-8984

The arrangement of histones along nucleosomal DNA within particular active and inactive genome regions was analysed by using protein-DNA crosslinking methods combined with hybridization tests. Two-dimensional gel electrophoresis was employed to compare the nucleosome conformation and nucleosomal DNA size. The arrangement of histones along DNA and general compactness of nucleosomes were shown to be very similar in transcriptionally active and inactive genomic regions. On the other hand, nucleosomes within transcriptionally active chromatin are characterized by a somewhat larger size of nucleosomal DNA produced by micrococcal nuclease digestion and some peculiarity in electrophoretic mobility. It appears that changes in transcribed chromatin structure (such as an enhanced nuclease sensitivity) occur on the supranucleosomal level rather than in the nucleosomal core structure.