The structure of nucleosomal core particles located on the transcribed genome regions

The arrangement of histones along nucleosomal DNA within particular active and inactive genome regions was analysed by using protein-DNA crosslinking methods combined with hybridization tests. Two-dimensional gel electrophoresis was employed to compare the nucleosome conformation and nucleosomal DNA...

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Published inMolekuliarnaia biologiia Vol. 22; no. 3; p. 706
Main Authors Studitskiĭ, V M, Beliavskiĭ, A V, Mel'nikova, A F, Mirzabekov, A D
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1988
Subjects
Animals
Drosophila melanogaster - genetics
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Heat-Shock Proteins - genetics
Histones - genetics
Histones - ultrastructure
Nucleosomes - analysis
Nucleosomes - ultrastructure
Transcription, Genetic
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Summary:The arrangement of histones along nucleosomal DNA within particular active and inactive genome regions was analysed by using protein-DNA crosslinking methods combined with hybridization tests. Two-dimensional gel electrophoresis was employed to compare the nucleosome conformation and nucleosomal DNA size. The arrangement of histones along DNA and general compactness of nucleosomes were shown to be very similar in transcriptionally active and inactive genomic regions. On the other hand, nucleosomes within transcriptionally active chromatin are characterized by a somewhat larger size of nucleosomal DNA produced by micrococcal nuclease digestion and some peculiarity in electrophoretic mobility. It appears that changes in transcribed chromatin structure (such as an enhanced nuclease sensitivity) occur on the supranucleosomal level rather than in the nucleosomal core structure.
ISSN:0026-8984