A new class of small RNP (alpha-RNP) containing antisense RNA in K-562 cells. III. The DNA-binding activity of nuclear alpha-RNP. The Signal Transmission from Growth Factors Group of the Medical Faculty, F. Schiller University, Jena, Germany

• Published in: Ontogenez Vol. 28; no. 3; p. 171
• Main Authors: Konstantinova, I M, Wetzker, R, Petukhova, O A, Chesnokov, I N, Volkova, I V, Turoverova, L V, Ermolaeva, Iu B, Gauze, L N
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.1997
• Subjects: Base Sequence; Cell Nucleus - drug effects; Cell Nucleus - genetics; Cell Nucleus - metabolism; Chromatin - drug effects; Chromatin - genetics; Chromatin - metabolism; Dimethyl Sulfoxide - pharmacology; DNA Probes; DNA, Neoplasm - drug effects; DNA, Neoplasm - genetics; DNA, Neoplasm - metabolism; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Neoplastic - genetics; Gene Expression Regulation, Neoplastic - physiology; Humans; Leukemia, Erythroblastic, Acute - genetics; Leukemia, Erythroblastic, Acute - metabolism; Molecular Sequence Data; Repetitive Sequences, Nucleic Acid - drug effects; Repetitive Sequences, Nucleic Acid - genetics; Repetitive Sequences, Nucleic Acid - physiology; Ribonucleoproteins, Small Nuclear - drug effects; Ribonucleoproteins, Small Nuclear - genetics; Ribonucleoproteins, Small Nuclear - metabolism; RNA, Antisense - drug effects; RNA, Antisense - genetics; RNA, Antisense - metabolism; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - metabolism
• ISSN: 0475-1450

DNA-binding activity of small nuclear alpha-RNP identified in acid-soluble fraction of chromatin of human proerythroleukemic cell line K-562 was studied using the technique of gel retardation. We found that nuclear alpha-RNP isolated from K-562 cells through treatment with dimethylsulfoxide, an agent inducing differentiation, acquire a capacity to specific interaction with Alu repeats of DNA leading to the formation of alpha-RNP-Alu-DNA complexes; nuclear alpha-RNP from cells that were not treated with dimethylsulfoxide do not show such capacity, although they are tightly bound with chromatin in the cell. Thus, the capacity of nuclear alpha-RNP to direct interaction with DNA Alu repeats appearing after the induction of K-562 cells to differentiation along erythroid pathway is an inducible property. We discuss hypothesis about the involvement of nuclear alpha-RNP in the control of expression of inducible genes at the level of chromatin and interaction with DNA.