Study on the conformational state of Escherichia coli tRNA-Phe in solution by ESR-spectometry without modulation
ESR-spectrometry without modulation of the magnetic field was used for registering the EST spectral line shape (with shape distortion about 0.1 percent) of spin-labeled Escherichia coli tRNAPhe. The analysis of line shape of two different spin-labels in position 8 (S4U) revealed that tRNAPhe in solu...
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Published in | Molekuliarnaia biologiia Vol. 16; no. 2; p. 352 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Russian |
Published |
Russia (Federation)
01.03.1982
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Subjects | |
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Abstract | ESR-spectrometry without modulation of the magnetic field was used for registering the EST spectral line shape (with shape distortion about 0.1 percent) of spin-labeled Escherichia coli tRNAPhe. The analysis of line shape of two different spin-labels in position 8 (S4U) revealed that tRNAPhe in solution always exists as a mixture of at least two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and the biological state of tRNA (deacylated, aminoacyl- or peptidyl-tRNA). There are no large structural rearrangements upon aminoacylation or peptidylation of tRNA, the observed small changes of spectral line shape are due to the changes in conformational equilibria. |
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AbstractList | ESR-spectrometry without modulation of the magnetic field was used for registering the EST spectral line shape (with shape distortion about 0.1 percent) of spin-labeled Escherichia coli tRNAPhe. The analysis of line shape of two different spin-labels in position 8 (S4U) revealed that tRNAPhe in solution always exists as a mixture of at least two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and the biological state of tRNA (deacylated, aminoacyl- or peptidyl-tRNA). There are no large structural rearrangements upon aminoacylation or peptidylation of tRNA, the observed small changes of spectral line shape are due to the changes in conformational equilibria. |
Author | Bondarev, G N Isaeva-Ivanova, L S Kleĭner, A R Kirillov, S V Isaev-Ivanov, V V |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/6175894$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Electron Spin Resonance Spectroscopy Escherichia coli - analysis Hydrogen-Ion Concentration Nucleic Acid Conformation RNA, Bacterial RNA, Transfer, Amino Acyl Solutions Spin Labels |
Title | Study on the conformational state of Escherichia coli tRNA-Phe in solution by ESR-spectometry without modulation |
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