Study on the conformational state of Escherichia coli tRNA-Phe in solution by ESR-spectometry without modulation

ESR-spectrometry without modulation of the magnetic field was used for registering the EST spectral line shape (with shape distortion about 0.1 percent) of spin-labeled Escherichia coli tRNAPhe. The analysis of line shape of two different spin-labels in position 8 (S4U) revealed that tRNAPhe in solu...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 16; no. 2; p. 352
Main Authors Bondarev, G N, Isaev-Ivanov, V V, Isaeva-Ivanova, L S, Kirillov, S V, Kleĭner, A R
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1982
Subjects
Electron Spin Resonance Spectroscopy
Escherichia coli - analysis
Hydrogen-Ion Concentration
Nucleic Acid Conformation
RNA, Bacterial
RNA, Transfer, Amino Acyl
Solutions
Spin Labels
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Summary:ESR-spectrometry without modulation of the magnetic field was used for registering the EST spectral line shape (with shape distortion about 0.1 percent) of spin-labeled Escherichia coli tRNAPhe. The analysis of line shape of two different spin-labels in position 8 (S4U) revealed that tRNAPhe in solution always exists as a mixture of at least two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and the biological state of tRNA (deacylated, aminoacyl- or peptidyl-tRNA). There are no large structural rearrangements upon aminoacylation or peptidylation of tRNA, the observed small changes of spectral line shape are due to the changes in conformational equilibria.
ISSN:0026-8984