The results of determining compatibility in the MLC reaction in hemoblastosis patients undergoing bone marrow transplantation from HLA-identical siblings

Compatibility in mixed lymphocyte culture (MLC) test was determined for 60 hemoblastosis patients with their 71 HLA-identical sibs to transplant allogenic syngeneic bone marrow. A protocol for the MLC radioisotope micromethod with culturing in plates is described for the pair donor-recipient in the...

Full description

Saved in:
Bibliographic Details
Published inGematologiia i transfuziologiia Vol. 38; no. 5; p. 17
Main Authors Shpakova, A P, Bulycheva, T I, Dronova, V M, Okhrimenko, T N, Liubimova, L S, Mendeleeva, L P, Savchenko, V G
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1993
Subjects
Bone Marrow Transplantation - immunology
Female
Genotype
Haplotypes - genetics
Haplotypes - immunology
HLA Antigens - blood
HLA Antigens - genetics
Humans
Immunophenotyping
Leukemia - genetics
Leukemia - immunology
Leukemia - therapy
Lymphocyte Culture Test, Mixed - methods
Lymphocyte Culture Test, Mixed - statistics & numerical data
Male
Transplantation, Homologous
Online AccessGet more information

Cover

More Information
Summary:Compatibility in mixed lymphocyte culture (MLC) test was determined for 60 hemoblastosis patients with their 71 HLA-identical sibs to transplant allogenic syngeneic bone marrow. A protocol for the MLC radioisotope micromethod with culturing in plates is described for the pair donor-recipient in the direct and reverse variants covering about 20 variants of mixed cultures. This secures the results' reliability. Plotting of the MLC compatibility results for each pair has been proposed. Incompatibility by MLC was revealed in 5 out of 71 pairs (7%). Bone marrow transplantation was performed in 30 patients from their HLA-identical/MLC-compatible sibs. The secondary disease due to incompatibility missed in the MLC reaction occurred in 5 cases (16.7%), transplant rejection in 2 cases (6.7%), which is less in number than literature data. It was established that determination of compatibility by MLC may serve a reliable criterion of assessing a genotypic pair HLA identity and should be conducted before each allogenic and syngeneic transplantation of bone marrow. The protocols of the method performance and compatibility parameters evaluation warrant high significance of the compatibility definition confirmed by clinical responses with minimal probability of secondary disease.
ISSN:0234-5730