Use of colloidal gold in ultrastructural cytochemistry
Colloid gold stabilized by macromolecules with various binding characteristics proved to be usable in transmission electron microscopy: Nucleic acids localization was studied in tissue cultures after embedding by techniques autoimmune serum-protein A--gold and RNase--gold. Technique with serum autoa...
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Published in | Ceskoslovenské patologie Vol. 21; no. 1; p. 28 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | Czech |
Published |
Czech Republic
01.02.1985
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Subjects | |
Online Access | Get more information |
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Summary: | Colloid gold stabilized by macromolecules with various binding characteristics proved to be usable in transmission electron microscopy: Nucleic acids localization was studied in tissue cultures after embedding by techniques autoimmune serum-protein A--gold and RNase--gold. Technique with serum autoantibodies against double-stranded DNA resulted in marking condensed chromatin. Further experiments with ultrastructural localization of other autoimmune components seemed to be perspective and of diagnostic importance. Technique with RNase--gold complex marked especially ribosomes, nucleolus and interchromatin nuclear spaces. Herpes virus, rotavirus and enterovirus were identified in negative contrast by the technique antivirus serum--protein A--gold. A higher warrant of evidence achieved by the method may be used in virologic diagnosis. A small amount of tubulin was found in isolated permeabilized nuclei of Xenopus laevis by a direct immunocytochemical method with complex monoclonal IgG against tubulin-gold. Binding of complex of triiodothyronine-bovine serum albumin--gold on the cytoplasmic membrane of LEP cells in a short term tissue culture showed a possibility of tracing non-peptidic hormones binding on specific receptors. |
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ISSN: | 1210-7875 |