Contractile proteins and nonerythroid spectrin in the oogenesis of the clawed toad

• Published in: Ontogenez Vol. 23; no. 5; p. 487
• Main Authors: Riabova, L V, Virtanen, I, Vartiovaara, J, Vasetskiĭ, S G
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.09.1992
• Subjects: Actins - metabolism; Animals; Contractile Proteins - metabolism; Electrophoresis, Polyacrylamide Gel; Female; Fluorescent Antibody Technique; Immunoblotting - methods; Membrane Proteins - metabolism; Myosins - metabolism; Oocytes - growth & development; Oocytes - metabolism; Oogenesis - physiology; Spectrin; Vitellogenesis - physiology; Xenopus laevis - physiology
• ISSN: 0475-1450

Distribution of contractile proteins, actin and myosin, and spectrin was studied in oogenesis of X. laevis. These proteins are present already at the previtellogenic stages, where they are diffusely distributed. During vitellogenesis actin and myosin are distributed in the animal region in a fibril-like way, while in the vegetal one they are concentrated around the yolk platelets. In the mature oocyte, distribution of actin and myosin again becomes diffuse. Spectrin forms in the vitellogenic oocyte a network all over the cytoplasm, while in the full-grown oocyte it is localized mostly in the subcortex of the animal region and disappears during oocyte maturation. All these proteins are present in the nuclei of oocytes. Changes in distribution of actin, myosin and spectrin during oocyte maturation are discussed with reference to the cortical contractility, spatial distribution of yolk platelets and regional sensitivity to cytochalasin B.