An evaluation of the Khramkova-Abelev immunodiffusion method for determining the lectin concentration in a solution

Using the method of double radial immunodiffusion with highly purified preparations of HRPO and lectins PSA, PNA, SNA the threshold sensitivity of precipitation in agar gel, 7.2 micrograms/ml, was established. The use of monospecific immunodiffusion test system for PNA helped increase the sensitivit...

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Bibliographic Details
Published inBiulleten' eksperimental'noi biologii i meditsiny Vol. 116; no. 12; p. 593
Main Authors Kalinin, Iu A, Tatarinov, Iu S, Terent'ev, A A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.12.1993
Subjects
Animals
Chi-Square Distribution
Evaluation Studies as Topic
Humans
Immunodiffusion - methods
Immunodiffusion - statistics & numerical data
Lectins - analysis
Lectins - isolation & purification
Plant Proteins - analysis
Plant Proteins - isolation & purification
Solutions
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Summary:Using the method of double radial immunodiffusion with highly purified preparations of HRPO and lectins PSA, PNA, SNA the threshold sensitivity of precipitation in agar gel, 7.2 micrograms/ml, was established. The use of monospecific immunodiffusion test system for PNA helped increase the sensitivity of immunodiffusion analysis to 3.6 micrograms/ml.
ISSN:0365-9615