A rapid technique for extraction of urinary 17-kéto and 17-hydroxy-corticosteroids by chromatography (author's transl)

The extraction ot total urinary 17-keto and 17-hydroxy steroids has been effected by a rapid procedure involving pre-packed kieselguhr column chromatography. This work successively studies the correlation of this technique with the classical extraction by mechanical agitation (42 and 61 subjects), i...

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Bibliographic Details
Published inPathologie biologie (Paris) Vol. 29; no. 8; p. 513
Main Authors Reboul-Salze, S, Enjolras, F, Gentou, C
Format Journal Article
LanguageFrench
Published France 01.10.1981
Subjects
17-Hydroxycorticosteroids - urine
17-Ketosteroids - urine
Chromatography, Liquid - methods
Humans
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Summary:The extraction ot total urinary 17-keto and 17-hydroxy steroids has been effected by a rapid procedure involving pre-packed kieselguhr column chromatography. This work successively studies the correlation of this technique with the classical extraction by mechanical agitation (42 and 61 subjects), its reproducibility, repeatability and recovery of added internal standards. The proposed method seems convenient for routine clinical analysis.
ISSN:0369-8114