The effect of 5-azacytidine on the cell cycle and chromosome structure in cell cultures of swine embryonal kidney tissue

Incubation of pig kidney cells (PK-cells) in the presence of 5-azacytidine (5-azaC), a DNA enzymatic methylation inhibitor, at concentration of 20 microM for 6 or 24 h results in a dramatic decrease in the DNA methylation level (5mC/C + m5.100) - from 3.0 in control to 1.0 in experiment. This is acc...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 23; no. 3; p. 772
Main Authors Zatsepina, O V, Aĭrapetian, V O, Kir'ianov, G I
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1989
Subjects
Animals
Azacitidine - pharmacology
Cell Cycle - drug effects
Cell Survival - drug effects
Chromatin - ultrastructure
Chromosomes - ultrastructure
Culture Techniques
DNA - analysis
DNA - drug effects
DNA Replication - drug effects
Kidney
Methylation
Mitotic Index - drug effects
Swine
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Summary:Incubation of pig kidney cells (PK-cells) in the presence of 5-azacytidine (5-azaC), a DNA enzymatic methylation inhibitor, at concentration of 20 microM for 6 or 24 h results in a dramatic decrease in the DNA methylation level (5mC/C + m5.100) - from 3.0 in control to 1.0 in experiment. This is accompanied by a virtually complete arrest of mitosis and a decrease in the ratio of labeled interphase cells upon simultaneous introduction of 3H-deoxycytidine. The incubation with 5-azaC block PK-cells mainly in the G2-period. The inhibitory action is reversible, for the cells enter into mitosis after removal of the inhibitor. Metaphase chromosomes, whose DNA was replicated in the presence of the 5-azaC, exhibit certain ultrastructural differences from normal ones. The results are being discussed in connection with the earlier data on the anomalous structure of interphase chromatin formed in the course undermethylated DNA replication.
ISSN:0026-8984