Effect of glycerin removal on the electrical resistance of isolated muscle fiber in the frog

• Published in: T͡S︡itologii͡a Vol. 26; no. 5; p. 610
• Main Authors: Shvinka, N E, Caffier, G
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.05.1984
• Subjects: Animals; Electric Conductivity; Glycerol - pharmacology; Hypertonic Solutions; In Vitro Techniques; Isotonic Solutions; Membrane Potentials - drug effects; Muscles - drug effects; Muscles - physiology; Rana esculenta; Sulfates - pharmacology; Time Factors
• ISSN: 0041-3771

Fibres isolated from iliofibularis muscles of the frog Rana esculenta were studied under current-clamp conditions with a double sucrose-gap technique. An increase of membrane resistance in muscle fibres (Rm) was demonstrated during the first 10-15 min of glycerol removal in K2SO4 solution. After a 30 min treatment in glycerol-containing K2SO4 Rm was 1.40 +/- 0.12 k omega X X cm2. The transfer of muscles from the glycerol-K2SO4 solution to an isotonic K2SO4 solution resulted in a progressive increase in the Rm which after a 15 minutes removal of glycerol reached the mean value of 2.02 +/- 0.22 k omega X cm2. It is suggested that this increase may reflect detubulation of the fibres caused by glycerol removal in muscles.