Measurement of salivary cortisol with four commercial kits

• Published in: Annales de biologie clinique (Paris) Vol. 54; no. 2; p. 75
• Main Authors: Nahoul, K, Patricot, M C, Bressot, N, Penes, M C, Revol, A
• Format: Journal Article
• Language: French
• Published: France 1996
• Subjects: Humans; Hydrocortisone - analysis; Immunoassay - methods; Reagent Kits, Diagnostic; Saliva - chemistry
• ISSN: 0003-3898

Salivary cortisol was determined with four commercially available immunoassays: one enzyme-immunoassay (Cortisol Biotrol), two fluoro-immunoassays (TDX, Abbott; Stratus, Baxter) and one radioimmunoassay (Coat-A-Count, Behring). In order to improve the sensitivity of these immunoassays, it was necessary to increase sample volumes. Thus an extraction step had to be included in the procedure. It was performed with either methylene chloride or Bond Elut. However, the Coat-A-Count kit may be applied directly on salivary aliquots. The results obtained were compared to those yielded by the reference technique which includes a chromatographic step on Sephadex LH 20. According to the present data (n = 20) no kit appears to be adequate for salivary cortisol measurement at any level, at least in the conditions applied in this study. However, the introduction of a chromatographic step in the procedure improved greatly the specificity. Nevertheless, the best results were obtained with the Coat-A-Count kit. Indeed, they were generally similar to those of the reference technique, but some discrepancies were observed at low levels.