Study of the vaccinating properties of 2 variants of Marek's disease virus

• Published in: Voprosy virusologiĭ no. 4; p. 493
• Main Authors: Iakovleva, L S, Merekalova, Z I, Pavlovskaia, A I, Plakhov, I V, Mazurenko, N P
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.07.1977
• Subjects: Animals; Chick Embryo; Chickens; Drug Evaluation, Preclinical; Genetic Variation; Herpesvirus 2, Gallid - immunology; Herpesvirus 2, Gallid - pathogenicity; Marek Disease - prevention & control; Vaccination; Vaccines, Attenuated - administration & dosage; Viral Vaccines - administration & dosage
• ISSN: 0507-4088

The vaccinating properties of two variants of Marek's disease virus, Kekava strain (MDV-Kekava) have been studied. One of them is naturally attenuated, the other has been produced as a result of attenuation of the pathogenic MDV-Kekava by long-term passages in chick embryo fibroblast (CEF) culture. The vaccinating properties of the naturally attenuated strains (MDV-Kekava 83) were first studied in an experiment in which the blood of chicken No. 1683 was used as the vaccine. In paralled. the MDV-Kekava 83 variant was isolated from the blood of this chicken in CEF culture. Vaccination of chickens with the blood of this chicken as well as with 1000-3000 PFU MDV-Kekava 83 which had undergone 16, 24, 45, and 46 passages in vitro produced in the chickens resistance to Marek's disease. According to the combined data of 4 experiments, altogether 9 out of 101 vaccinated chickens developed Marek's disease. Among the controls inoculated with the pathogenic MDV alone 51 out of 137 chickens developed the disease. Attenuation of the pathogenic MDV-Kekava 55 in CEF culture occurred very slowly. The virus of the 45th passage in vitro had no vaccinating properties. Vaccination of the chickens with 1000-2500 PFU MDV-Kekava 56 which had undergone 73 a94 passages in CEF culture produced resistance to Marek's disease. Among the vaccinated chickens 20 out of 113 had Marek's disease whereas in the control group 74 out of 122 were observed to develop the disease. Among the chickens vaccinated with MDV, 3-4% developed the disease. Possible causes of the disease in this group of chickens are discussed.