TREX1 is expressed by microglia in normal human brain and increases in regions affected by ischemia

• Published in: Brain pathology (Zurich, Switzerland) Vol. 28; no. 6; pp. 806 - 821
• Main Authors: Kothari, Parul H., Kolar, Grant R., Jen, Joanna C., Hajj‐Ali, Rula, Bertram, Paula, Schmidt, Robert E., Atkinson, John P.
• Format: Journal Article
• Language: English
• Published: Switzerland John Wiley & Sons, Inc 01.11.2018; John Wiley and Sons Inc
• Subjects: Aged; Animals; Antibodies - metabolism; Brain; Brain - metabolism; Brain - pathology; brain ischemia; Brain Ischemia - metabolism; Brain Ischemia - pathology; Central nervous system; Cerebral cortex; DNase1; Exodeoxyribonucleases - genetics; Exodeoxyribonucleases - metabolism; Exodeoxyribonucleases - physiology; Exonuclease; Female; Frameshift Mutation; HEK293 Cells; HeLa Cells; Hereditary Central Nervous System Demyelinating Diseases - genetics; Hereditary Central Nervous System Demyelinating Diseases - pathology; Homeostasis; Homeostasis - physiology; Humans; Ischemia; Lesions; Leukoencephalopathy; Lysates; Macrophages; Macrophages - metabolism; Male; Microglia; Microglia - metabolism; Microvasculature; Middle Aged; Mutation; Neurological diseases; Paraffin; Paraffins; Patients; Phosphoproteins - genetics; Phosphoproteins - metabolism; Phosphoproteins - physiology; Polyclonal antibodies; Rabbits - immunology; Retina; Retinal Diseases - genetics; Retinal Diseases - pathology; Staining; Stroke; Substantia alba; Substantia grisea; TREX1; Vascular diseases; Vascular Diseases - genetics; Vascular Diseases - pathology; vasculopathy; Western blotting; macrophages; DNase1; brain ischemia; vasculopathy; microglia; TREX1
• ISSN: 1015-6305; 1750-3639; 1750-3639
• DOI: 10.1111/bpa.12626

Background Mutations in the three‐prime repair exonuclease 1 (TREX1) gene have been associated with neurological diseases, including Retinal Vasculopathy with Cerebral Leukoencephalopathy (RVCL). However, the endogenous expression of TREX1 in human brain has not been studied. Methods We produced a r...