Investigation of H2AX methylation by the SUV39H2 protein lysine methyltransferase

• Published in: FEBS letters Vol. 590; no. 12; pp. 1713 - 1719
• Main Authors: Schuhmacher, Maren Kirstin, Kudithipudi, Srikanth, Jeltsch, Albert
• Format: Journal Article
• Language: English
• Published: England 01.06.2016
• Subjects: DNA repair; enzyme specificity; H2AX; Histone-Lysine N-Methyltransferase - chemistry; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - metabolism; Histones - chemistry; Histones - genetics; Histones - metabolism; Humans; Methylation; peptide recognition; protein lysine methylation; Substrate Specificity; SUV39H2; H2AX; SUV39H2; peptide recognition; enzyme specificity; protein lysine methylation; DNA repair

The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX‐K134 differs from the specificity of SUV39H2. We performed in vitro...