Transforming Growth Factor Beta 1 Induces Tight Junction Disruptions and Loss of Transepithelial Resistance Across Porcine Vas Deferens Epithelial Cells

• Published in: Biology of reproduction Vol. 86; no. 2; p. 36
• Main Authors: PIERUCCI-ALVES, Fernando, YI, Sheng, SCHULTZ, Bruce D
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.02.2012; Society for the Study of Reproduction, Inc
• Subjects: Animals; Anions; Biological and medical sciences; Cell culture; Cell Membrane Permeability - drug effects; Cell Membrane Permeability - physiology; Cells, Cultured; Claudins - metabolism; Cytokines; Data processing; Diffusion; Electrophysiological Phenomena; Epithelial cells; Epithelial Cells - drug effects; Epithelial Cells - physiology; Epithelium; Fertility; Forskolin; Fundamental and applied biological sciences. Psychology; Male; MAP kinase; Membrane Proteins - metabolism; Microenvironments; Mitogen-Activated Protein Kinase 11 - antagonists & inhibitors; Models, Animal; Occludin; Patch-Clamp Techniques; Receptor mechanisms; Semen; Solutes; Sperm; Swine; Tight junctions; Tight Junctions - drug effects; Tight Junctions - metabolism; Transforming Growth Factor beta1 - pharmacology; Transforming growth factor- beta; Transforming growth factor- beta 1; Vas deferens; Vas Deferens - cytology; Vertebrates: reproduction; Semen pathway; growth factors; Transforming growth factor; Cytokine; Cell junction; Male; Male genital duct; Male genital system; Vas deferens; Pig; Vertebrata; Reproduction; Mammalia; Animal; Epithelial cell; Tight junction; cytokines; Artiodactyla; male epithelial barrier; male reproductive tract; Ungulata; Growth factor

Epithelial cells lining the male excurrent duct contribute to male fertility by employing a number of physiological mechanisms that generate a luminal microenvironment conducive to spermatozoa maturation and storage. Among these mechanisms, male duct epithelia establish intercellular tight junctions...