The CrdRS two‐component system in Helicobacter pylori responds to nitrosative stress
Summary Helicobacter pylori inhabits the gastric mucosa where it senses and responds to various stresses via a two‐component systems (TCSs) that enable its persistent colonization. The aim of this study was to investigate whether any of the three paired TCSs (ArsRS, FleRS and CrdRS) in H. pylori res...
Saved in:
Published in | Molecular microbiology Vol. 97; no. 6; pp. 1128 - 1141 |
---|---|
Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Blackwell Publishing Ltd
01.09.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Summary
Helicobacter pylori inhabits the gastric mucosa where it senses and responds to various stresses via a two‐component systems (TCSs) that enable its persistent colonization. The aim of this study was to investigate whether any of the three paired TCSs (ArsRS, FleRS and CrdRS) in H. pylori respond to nitrosative stress. The results showed that the expression of crdS was significantly increased upon exposure to nitric oxide (NO). crdS‐knockout (ΔcrdS) and crdR/crdS‐knockout (ΔcrdRS) H. pylori, but not arsS‐knockout (ΔarsS) or fleS‐knockout (ΔfleS) H. pylori, showed a significant loss of viability upon exposure to NO compared with wild‐type strain. Knockin crdS (ΔcrdS‐in) significantly restored viability in the presence of NO. Global transcriptional profiling analysis of wild‐type and ΔcrdS H. pylori in the presence or absence of NO showed that 101 genes were differentially expressed, including copper resistance determinant A (crdA), transport, binding and envelope proteins. The CrdR binding motifs were investigated by competitive electrophoretic mobility shift assay, which revealed that the two AC‐rich regions in the crdA promoter region are required for binding. These results demonstrate that CrdR–crdA interaction enables H. pylori to survive under nitrosative stress.
Helicobacter pylori successfully responds and adapts to various stresses from gastric environment via its three‐pair two‐component systems (TCSs). This report shows that a unique TCS, CrdRS, functions as a sensor for nitrosative stress. Nitrosative challenge differentially upregulates the expression of resistance determinant A (crdA). Further, CrdR binds to the proximal promoter region of crdA that consists of a two AC‐rich region. These findings demonstrate that CrdR‐crdA interaction enables H. pylori to survive under nitrosative stress. |
---|---|
AbstractList | Summary
Helicobacter pylori inhabits the gastric mucosa where it senses and responds to various stresses via a two‐component systems (TCSs) that enable its persistent colonization. The aim of this study was to investigate whether any of the three paired TCSs (ArsRS, FleRS and CrdRS) in H. pylori respond to nitrosative stress. The results showed that the expression of crdS was significantly increased upon exposure to nitric oxide (NO). crdS‐knockout (ΔcrdS) and crdR/crdS‐knockout (ΔcrdRS) H. pylori, but not arsS‐knockout (ΔarsS) or fleS‐knockout (ΔfleS) H. pylori, showed a significant loss of viability upon exposure to NO compared with wild‐type strain. Knockin crdS (ΔcrdS‐in) significantly restored viability in the presence of NO. Global transcriptional profiling analysis of wild‐type and ΔcrdS H. pylori in the presence or absence of NO showed that 101 genes were differentially expressed, including copper resistance determinant A (crdA), transport, binding and envelope proteins. The CrdR binding motifs were investigated by competitive electrophoretic mobility shift assay, which revealed that the two AC‐rich regions in the crdA promoter region are required for binding. These results demonstrate that CrdR–crdA interaction enables H. pylori to survive under nitrosative stress.
Helicobacter pylori successfully responds and adapts to various stresses from gastric environment via its three‐pair two‐component systems (TCSs). This report shows that a unique TCS, CrdRS, functions as a sensor for nitrosative stress. Nitrosative challenge differentially upregulates the expression of resistance determinant A (crdA). Further, CrdR binds to the proximal promoter region of crdA that consists of a two AC‐rich region. These findings demonstrate that CrdR‐crdA interaction enables H. pylori to survive under nitrosative stress. Helicobacter pylori inhabits the gastric mucosa where it senses and responds to various stresses via a two-component systems (TCSs) that enable its persistent colonization. The aim of this study was to investigate whether any of the three paired TCSs (ArsRS, FleRS and CrdRS) in H. pylori respond to nitrosative stress. The results showed that the expression of crdS was significantly increased upon exposure to nitric oxide (NO). crdS-knockout (...crdS) and crdR/crdS-knockout (...crdRS) H. pylori, but not arsS-knockout (...arsS) or fleS-knockout (...fleS) H. pylori, showed a significant loss of viability upon exposure to NO compared with wild-type strain. Knockin crdS (...crdS-in) significantly restored viability in the presence of NO. Global transcriptional profiling analysis of wild-type and ...crdS H. pylori in the presence or absence of NO showed that 101 genes were differentially expressed, including copper resistance determinant A (crdA), transport, binding and envelope proteins. The CrdR binding motifs were investigated by competitive electrophoretic mobility shift assay, which revealed that the two AC-rich regions in the crdA promoter region are required for binding. These results demonstrate that CrdR-crdA interaction enables H. pylori to survive under nitrosative stress. (ProQuest: ... denotes formulae/symbols omitted.) Helicobacter pylori inhabits the gastric mucosa where it senses and responds to various stresses via a two-component systems (TCSs) that enable its persistent colonization. The aim of this study was to investigate whether any of the three paired TCSs (ArsRS, FleRS and CrdRS) in H. pylori respond to nitrosative stress. The results showed that the expression of crdS was significantly increased upon exposure to nitric oxide (NO). crdS-knockout (ΔcrdS) and crdR/crdS-knockout (ΔcrdRS) H. pylori, but not arsS-knockout (ΔarsS) or fleS-knockout (ΔfleS) H. pylori, showed a significant loss of viability upon exposure to NO compared with wild-type strain. Knockin crdS (ΔcrdS-in) significantly restored viability in the presence of NO. Global transcriptional profiling analysis of wild-type and ΔcrdS H. pylori in the presence or absence of NO showed that 101 genes were differentially expressed, including copper resistance determinant A (crdA), transport, binding and envelope proteins. The CrdR binding motifs were investigated by competitive electrophoretic mobility shift assay, which revealed that the two AC-rich regions in the crdA promoter region are required for binding. These results demonstrate that CrdR-crdA interaction enables H. pylori to survive under nitrosative stress. |
Author | Wang, Wen‐Ching Chu, Chia‐Han Tsai, Zing Tsung‐Yeh Hsieh, Wan‐Chen Cheng, Hsin‐Hung Tsai, Huai‐Kuang Chen, Yi‐Fan Tsou, Yu Hsieh, Wen‐Ping Lai, Chih‐Ho Hung, Chiu‐Lien |
Author_xml | – sequence: 1 givenname: Chiu‐Lien surname: Hung fullname: Hung, Chiu‐Lien organization: National Tsing Hua University – sequence: 2 givenname: Hsin‐Hung surname: Cheng fullname: Cheng, Hsin‐Hung organization: National Tsing Hua University – sequence: 3 givenname: Wan‐Chen surname: Hsieh fullname: Hsieh, Wan‐Chen organization: National Tsing Hua University – sequence: 4 givenname: Zing Tsung‐Yeh surname: Tsai fullname: Tsai, Zing Tsung‐Yeh organization: Academia Sinica – sequence: 5 givenname: Huai‐Kuang surname: Tsai fullname: Tsai, Huai‐Kuang organization: Academia Sinica – sequence: 6 givenname: Chia‐Han surname: Chu fullname: Chu, Chia‐Han organization: National Tsing Hua University – sequence: 7 givenname: Wen‐Ping surname: Hsieh fullname: Hsieh, Wen‐Ping organization: National Tsing Hua University – sequence: 8 givenname: Yi‐Fan surname: Chen fullname: Chen, Yi‐Fan organization: National Tsing Hua University – sequence: 9 givenname: Yu surname: Tsou fullname: Tsou, Yu organization: National Tsing Hua University – sequence: 10 givenname: Chih‐Ho surname: Lai fullname: Lai, Chih‐Ho organization: Chang Gung University – sequence: 11 givenname: Wen‐Ching surname: Wang fullname: Wang, Wen‐Ching organization: National Tsing Hua University |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26082024$$D View this record in MEDLINE/PubMed |
BookMark | eNpd0c1KAzEQB_AgitaPgy8gAS9eVpPNJtkcpagttAhaxduS3U4xsrtZk9SyNx_BZ_RJTOvHwVwmMD-GYf77aLu1LSB0TMk5je-iacw5ZSRXW2hAmeBJqni-jQZEcZKwPH3aQ_vevxASkWC7aC8VJE9Jmg3Q4-wZ8NDN7-5xWNnP94_KNl2c3gbsex-gwabFI6hNZUtdBXC462vrDHbgo5t7HCxuTXDW62DeAPsQO_4Q7Sx07eHopx6gh-ur2XCUTG5vxsPLSdIxmqmEakEI4wutSFXyMlVE0TKvOKcMuJSSglBskWVA8rkogWoqpCZCyjLXDARhB-jse27n7OsSfCga4yuoa92CXfqCSko5kywVkZ7-oy926dq43VoxrgSXWVQnP2pZNjAvOmca7fri92IRXHyDlamh_-tTUqyjKGIUxSaKYjodbz7sC0l_fOI |
ContentType | Journal Article |
Copyright | 2015 John Wiley & Sons Ltd 2015 John Wiley & Sons Ltd. Copyright Blackwell Publishing Ltd. Sep 2015 |
Copyright_xml | – notice: 2015 John Wiley & Sons Ltd – notice: 2015 John Wiley & Sons Ltd. – notice: Copyright Blackwell Publishing Ltd. Sep 2015 |
DBID | CGR CUY CVF ECM EIF NPM 7QL 7QP 7QR 7TK 7TM 7U9 8FD C1K FR3 H94 M7N P64 RC3 7X8 |
DOI | 10.1111/mmi.13089 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Virology and AIDS Abstracts Technology Research Database Nucleic Acids Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Genetics Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts MEDLINE - Academic |
DatabaseTitleList | Virology and AIDS Abstracts MEDLINE - Academic MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1365-2958 |
EndPage | 1141 |
ExternalDocumentID | 3810594481 26082024 MMI13089 |
Genre | article Research Support, Non-U.S. Gov't Journal Article Feature |
GrantInformation_xml | – fundername: Ministry of Education and Tomorrow Medicine Foundation funderid: 102‐2325‐B‐007‐001 – fundername: Ministry of Science and Technology, Taiwan funderid: 98‐2627‐B‐007‐013; 99‐2627‐B‐007‐012; 100‐2627‐B‐007‐007; 101‐2313‐B‐039‐004‐MY3; 103‐2633‐B‐039‐001 |
GroupedDBID | --- -DZ .3N .55 .GA .GJ .HR .Y3 05W 0R~ 10A 123 1OB 1OC 24P 29M 2WC 31~ 33P 36B 3SF 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5HH 5LA 5RE 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHHS AAKAS AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABEML ABJNI ABPVW ABTAH ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACGFO ACGFS ACIWK ACNCT ACPOU ACPRK ACSCC ACXBN ACXQS ADBBV ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZCM ADZMN AEEZP AEGXH AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFEBI AFFPM AFGKR AFPWT AFRAH AFZJQ AHBTC AHEFC AIAGR AITYG AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AUFTA AVWKF AZBYB AZFZN AZVAB BAFTC BAWUL BDRZF BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BY8 C45 CAG COF D-E D-F DC6 DCZOG DIK DPXWK DR2 DRFUL DRSTM E3Z EBS EJD EMOBN ESX EX3 F00 F01 F04 F5P FEDTE FIJ FSRTE FZ0 G-S G.N GODZA GX1 H.T H.X HF~ HGLYW HH5 HVGLF HZI HZ~ IH2 IHE IPNFZ IX1 J0M K48 LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MVM MXFUL MXSTM N04 N05 N9A NF~ O66 O9- OBC OBS OEB OIG OK1 OVD P2P P2W P2X P4D PALCI PQQKQ Q.N Q11 QB0 R.K RIWAO RJQFR ROL RX1 SAMSI SUPJJ TEORI TR2 UB1 V8K W8V W99 WBKPD WH7 WIH WIK WIN WNSPC WOHZO WOW WQJ WRC WUP WXSBR WYISQ X7M XG1 Y6R YFH YUY ZGI ZXP ZY4 ZZTAW ~IA ~KM ~WT CGR CUY CVF ECM EIF NPM 7QL 7QP 7QR 7TK 7TM 7U9 8FD C1K FR3 H94 M7N P64 RC3 7X8 |
ID | FETCH-LOGICAL-p3149-1a60035fa90cb5b29091b8c5513e57771e693f44e08d6be1a167a0677b8a3e603 |
IEDL.DBID | DR2 |
ISSN | 0950-382X |
IngestDate | Sat Aug 17 01:35:05 EDT 2024 Fri Sep 13 02:42:11 EDT 2024 Sat Sep 28 07:56:30 EDT 2024 Sat Aug 24 00:40:39 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Language | English |
License | 2015 John Wiley & Sons Ltd. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-p3149-1a60035fa90cb5b29091b8c5513e57771e693f44e08d6be1a167a0677b8a3e603 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/mmi.13089 |
PMID | 26082024 |
PQID | 1713596574 |
PQPubID | 35968 |
PageCount | 14 |
ParticipantIDs | proquest_miscellaneous_1711537326 proquest_journals_1713596574 pubmed_primary_26082024 wiley_primary_10_1111_mmi_13089_MMI13089 |
PublicationCentury | 2000 |
PublicationDate | September 2015 2015-Sep 20150901 |
PublicationDateYYYYMMDD | 2015-09-01 |
PublicationDate_xml | – month: 09 year: 2015 text: September 2015 |
PublicationDecade | 2010 |
PublicationPlace | England |
PublicationPlace_xml | – name: England – name: Oxford |
PublicationTitle | Molecular microbiology |
PublicationTitleAlternate | Mol Microbiol |
PublicationYear | 2015 |
Publisher | Blackwell Publishing Ltd |
Publisher_xml | – name: Blackwell Publishing Ltd |
References | 2010; 34 2006; 74 2013; 3 2007; 282 2006; 34 2006; 57 2007; 162 2006; 79 2000; 68 2002; 7 2002; 2 2008; 105 2008; 76 2012; 18 2010; 184 2007; 75 2003; 71 2013; 8 2001; 25 2011; 193 1997; 388 2009; 77 2004; 52 2004; 53 1993; 139 2004; 234 2005; 187 2009; 191 2006; 22 2000; 54 2000; 97 2000; 182 2005; 73 1980; 8 1996; 111 2009; 284 2006; 126 1999; 116 2005; 33 2001; 98 |
References_xml | – volume: 18 start-page: 406 year: 2012 end-page: 417 article-title: attenuates lipopolysaccharide‐induced nitric oxide production by murine macrophages publication-title: Innate Immun – volume: 193 start-page: 40 year: 2011 end-page: 51 article-title: A cis‐encoded antisense small RNA regulated by the HP0165‐HP0166 two‐component system controls expression of in publication-title: J Bacteriol – volume: 3 start-page: 27 year: 2013 end-page: 33 article-title: Manipulation of host cholesterol by for their beneficial ecological niche publication-title: Biomedicine – volume: 34 start-page: D407 year: 2006 end-page: D410 article-title: The UCSC archaeal genome browser publication-title: Nucleic Acids Res – volume: 105 start-page: 2527 year: 2008 end-page: 2532 article-title: Discovering gapped binding sites of yeast transcription factors publication-title: Proc Natl Acad Sci USA – volume: 76 start-page: 3293 year: 2008 end-page: 3303 article-title: Cholesterol depletion reduces CagA translocation and CagA‐induced responses in AGS cells publication-title: Infect Immun – volume: 111 start-page: 1524 year: 1996 end-page: 1533 article-title: stimulates inducible nitric oxide synthase expression and activity in a murine macrophage cell line publication-title: Gastroenterology – volume: 284 start-page: 6536 year: 2009 end-page: 6545 article-title: Structural analysis of the DNA‐binding domain of the i response regulator ArsR publication-title: J Biol Chem – volume: 74 start-page: 602 year: 2006 end-page: 614 article-title: Iron and pH homeostasis intersect at the level of Fur regulation in the gastric pathogen Helicobacter pylori publication-title: Infect Immun – volume: 74 start-page: 3052 year: 2006 end-page: 3059 article-title: Requirement of histidine kinases HP0165 and HP1364 for acid resistance in publication-title: Infect Immun – volume: 34 start-page: 738 year: 2010 end-page: 752 article-title: Regulatory circuits in : network motifs and regulators involved in metal‐dependent responses publication-title: FEMS Microbiol Rev – volume: 98 start-page: 13844 year: 2001 end-page: 13849 article-title: arginase inhibits nitric oxide production by eukaryotic cells: a strategy for bacterial survival publication-title: Proc Natl Acad Sci USA – volume: 191 start-page: 449 year: 2009 end-page: 460 article-title: The pH‐responsive regulon of HP0244 (FlgS), the cytoplasmic histidine kinase of publication-title: J Bacteriol – volume: 75 start-page: 4305 year: 2007 end-page: 4315 article-title: L‐arginine availability regulates inducible nitric oxide synthase‐dependent host defense against publication-title: Infect Immun – volume: 97 start-page: 8841 year: 2000 end-page: 8848 article-title: Reactive oxygen and nitrogen intermediates in the relationship between mammalian hosts and microbial pathogens publication-title: Proc Natl Acad Sci USA – volume: 139 start-page: 2485 year: 1993 end-page: 2493 article-title: Transformation of by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker publication-title: J Gen Microbiol – volume: 77 start-page: 2590 year: 2009 end-page: 2601 article-title: This is not your mother's repressor: the complex role of fur in pathogenesis publication-title: Infect Immun – volume: 182 start-page: 5274 year: 2000 end-page: 5277 article-title: Mutational analysis of genes encoding the early flagellar components of : evidence for transcriptional regulation of flagellin A biosynthesis publication-title: J Bacteriol – volume: 52 start-page: 947 year: 2004 end-page: 961 article-title: Genome‐wide analysis of transcriptional hierarchy and feedback regulation in the flagellar system of publication-title: Mol Microbiol – volume: 73 start-page: 6437 year: 2005 end-page: 6445 article-title: Acid‐induced activation of the urease promoters is mediated directly by the ArsRS two‐component system of publication-title: Infect Immun – volume: 71 start-page: 2643 year: 2003 end-page: 2655 article-title: Gene expression profiling of reveals a growth‐phase‐dependent switch in virulence gene expression publication-title: Infect Immun – volume: 126 start-page: 52 year: 2006 end-page: 60 article-title: Acid‐responsive gene regulation in the human pathogen publication-title: J Biotechnol – volume: 25 start-page: 402 year: 2001 end-page: 408 article-title: Analysis of relative gene expression data using real‐time quantitative PCR and the 2(−Delta Delta C(T)) method publication-title: Methods – volume: 2 start-page: 147 year: 2002 end-page: 152 article-title: : 20 years on publication-title: Clin Med – volume: 187 start-page: 3687 year: 2005 end-page: 3692 article-title: Iron‐responsive regulation of the iron‐cofactored superoxide dismutase SodB is mediated by Fur publication-title: J Bacteriol – volume: 54 start-page: 881 year: 2000 end-page: 941 article-title: Iron metabolism in pathogenic bacteria publication-title: Annu Rev Microbiol – volume: 79 start-page: 1214 year: 2006 end-page: 1225 article-title: Role of urease in megasome formation and survival in macrophages publication-title: J Leukoc Biol – volume: 116 start-page: 1319 year: 1999 end-page: 1329 article-title: Increased expression and cellular localization of inducible nitric oxide synthase and cyclooxygenase 2 in gastritis publication-title: Gastroenterology – volume: 33 start-page: 880 year: 2005 end-page: 892 article-title: A novel method for accurate operon predictions in all sequenced prokaryotes publication-title: Nucleic Acids Res – volume: 187 start-page: 4683 year: 2005 end-page: 4688 article-title: The CrdRS two‐component regulation system (HP1364/HP1365) is required for copper‐mediated induction of the copper resistance determinant CrdA publication-title: J Bacteriol – volume: 184 start-page: 2572 year: 2010 end-page: 2582 article-title: Arginase II restricts host defense to by attenuating inducible nitric oxide synthase translation in macrophages publication-title: J Immunol – volume: 234 start-page: 51 year: 2004 end-page: 61 article-title: Genetic evidence for histidine kinase HP165 being an acid sensor of publication-title: FEMS Microbiol Lett – volume: 22 start-page: 1675 year: 2006 end-page: 1681 article-title: Method for identifying transcription factor binding sites in yeast publication-title: Bioinformatics – volume: 71 start-page: 5381 year: 2003 end-page: 5385 article-title: Two‐component systems of contribute to virulence in a mouse infection model publication-title: Infect Immun – volume: 53 start-page: 623 year: 2004 end-page: 638 article-title: Responsiveness to acidity via metal ion regulators mediates virulence in the gastric pathogen publication-title: Mol Microbiol – volume: 162 start-page: 1 year: 2007 end-page: 14 article-title: Regulation of expression of atypical orphan response regulators of publication-title: Microbiol Res – volume: 2 start-page: 28 year: 2002 end-page: 37 article-title: and gastrointestinal tract adenocarcinomas publication-title: Nat Rev Cancer – volume: 8 start-page: 725 year: 2013 end-page: 738 article-title: The ferric uptake regulator of : a critical player in the battle for iron and colonization of the stomach publication-title: Future Microbiol – volume: 388 start-page: 539 year: 1997 end-page: 547 article-title: The complete genome sequence of the gastric pathogen publication-title: Nature – volume: 68 start-page: 2016 year: 2000 end-page: 2023 article-title: possesses two CheY response regulators and a histidine kinase sensor, CheA, which are essential for chemotaxis and colonization of the gastric mucosa publication-title: Infect Immun – volume: 182 start-page: 2068 year: 2000 end-page: 2076 article-title: Molecular characterization of two‐component systems of publication-title: J Bacteriol – volume: 282 start-page: 20667 year: 2007 end-page: 20675 article-title: Structure of an atypical orphan response regulator protein supports a new phosphorylation‐independent regulatory mechanism publication-title: J Biol Chem – volume: 7 start-page: 237 year: 2002 end-page: 244 article-title: The role of the Ferric Uptake Regulator (Fur) in regulation of iron uptake publication-title: Helicobacter – volume: 8 start-page: 6081 year: 1980 end-page: 6097 article-title: Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin‐induced drug resistance publication-title: Nucleic Acids Res – volume: 57 start-page: 466 year: 2006 end-page: 471 article-title: Association of antibiotic resistance and higher internalization activity in resistant isolates publication-title: J Antimicrob Chemother |
SSID | ssj0013063 |
Score | 2.2973814 |
Snippet | Summary
Helicobacter pylori inhabits the gastric mucosa where it senses and responds to various stresses via a two‐component systems (TCSs) that enable its... Helicobacter pylori inhabits the gastric mucosa where it senses and responds to various stresses via a two-component systems (TCSs) that enable its persistent... |
SourceID | proquest pubmed wiley |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 1128 |
SubjectTerms | Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Copper - metabolism DNA-Binding Proteins - metabolism Gene expression Gene Expression Profiling Gene Knockout Techniques Gram-negative bacteria Helicobacter pylori - genetics Helicobacter pylori - metabolism Molecular Sequence Data Nitric oxide Nitric Oxide - metabolism Promoter Regions, Genetic Proteins Stress, Physiological |
Title | The CrdRS two‐component system in Helicobacter pylori responds to nitrosative stress |
URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fmmi.13089 https://www.ncbi.nlm.nih.gov/pubmed/26082024 https://www.proquest.com/docview/1713596574/abstract/ https://search.proquest.com/docview/1711537326 |
Volume | 97 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LSwMxEA6lIHjx_ahWieDBy5bdbpLdxZMUiwr1UB_0ICxJNoUi3ZZ2i-jJn-Bv9Jc4k2zrAw_ibSEJ5DGT-WYz8w0hx0kgObQkHhNYwizk2pM6NF7ENABYlfkiwUThzrW4uGNXPd6rkNN5Lozjh1j8cEPNsPc1KrhU0y9KPhwOsJRxjMl7SKSHgKjb_HxBKKuoJRz5Y5u9klUIo3gWI39Dld9BqrUy7VXyMJ-fCy55bMwK1dAvP6gb_7mANbJSok965sRlnVRMvkGWXD3K501yD0JDW5Ose0OLp9H76xsGnI9ysEvUMT7TQU7BUIH0KMvyTMfo7w_oxEbaZlNajCjcEWB5LZ84dZkoW-SufX7buvDKwgveOASPyQukwBfGvkx8rbhqJgAqVKyxFozhURQFRiRhnzHjx5lQJpCBiCRS0alYhkb44Tap5jC7XUIZUzLWmY7BBWeR4DIWffCRQu7LOOBa1Eh9fgRpqT3TNMC6gYngEauRo0UzyD0-ZsjcjGa2D1zWEaDPGtlxR5eOHUFHCj4aAJsmjD6xB7BomHs8sPWp3fq007m0H3t_77pPlgE1cRdoVifVYjIzB4BMCnVoRfADX0fexw |
link.rule.ids | 315,786,790,1382,27957,27958,46329,46753 |
linkProvider | Wiley-Blackwell |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1JSywxEC58iujF7bmMawQP79LSPZ2kO-BFRBmX8eDGXKRJ0hkYxJ5h7EH05E_wN_pLrCQ944IHebeGJJBOVaW-SipfAeyISDJsEQHltoRZzHQgdWyChGoEsCoPubAPhZvnvHFNT1qsNQZ7w7cwnh9idOBmLcPt19bA7YH0Jyu_v-_YWsap-AMTaO7MBVQX9Y87hKqOmmCWQbbeqniFbB7PaOhPuPIrTHV-5mgWbocz9Okld7uDUu3q52_kjf_7C3MwUwFQsu81Zh7GTLEAk74k5dNfuEG9IQf9_OKSlI_dt5dXm3PeLdA1EU_6TDoFQV-FCqQc0TPp2ZC_Q_ou2TZ_IGWX4DaBztdRihP_GGURro8Orw4aQVV7IejFGDQFkeT2krEtRagVU3WBuEKl2paDMSxJkshwEbcpNWGac2UiGfFEWjY6lcrY8DBegvECZ7cChFIlU53rFKNwmnAmU97GMClmoUwjpnkN1ocyyCoDesgiWzpQcJbQGmyPmlH17X2GLEx34Prgfp0gAK3Bspdd1vMcHRmGaYht6jj6n5PAqGEY9ODSZ27ps2bz2H2s_r7rFkw1rppn2dnx-ekaTCOIYj7vbB3Gy_7AbCBQKdWm08d3Y3ri6Q |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LbxMxEB6lQVRceLWF0FCMxIHLVvuwvWtxqhKi9JGoCrTKAWllex0pQtlEyUYITvwEfmN_CWN7kwLiUPW2kteS157xfLMefx_AOxFJhi0ioNxKmCVMB1InJkipRgCripALe1F4MOT9K3o2ZuMGfNjchfH8ENsfbtYz3H5tHXxRTP5w8tlsaqWMM7EDDyhPYmvS3VF8e4RQy6gJZglk43FNK2TLeLZd_wcr_0apLsz0nsCXzQB9dcnX43WljvWPf7gb7_kFT-FxDT_JibeXZ9Aw5XN46AUpv-_BNVoN6SyL0SdSfZvf_PxlK87nJQYm4imfybQkGKnQfJSjeSYLm_BPydKV2hYrUs0JbhIYeh2hOPFXUfbhqvfxc6cf1MoLwSLBlCmIJLdHjBMpQq2YigWiCpVpKwZjWJqmkeEimVBqwqzgykQy4qm0XHQqk4nhYXIAzRJH9xIIpUpmutAZ5uA05UxmfIJJUsJCmUVM8xa0N0uQ1-6zyiMrHCg4S2kL3m6b0fDtaYYszXzt3sHdOkX42YIXfunyhWfoyDFJQ2QTY-_3bgG2DZuUB6c-d1OfDwan7uHV3V99A7uX3V5-cTo8P4RHiKCYLzprQ7Nars1rRCmVOnLW-BvXwOGY |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+CrdRS+two-component+system+in+Helicobacter+pylori+responds+to+nitrosative+stress&rft.jtitle=Molecular+microbiology&rft.au=Hung%2C+Chiu-Lien&rft.au=Cheng%2C+Hsin-Hung&rft.au=Hsieh%2C+Wan-Chen&rft.au=Tsai%2C+Zing+Tsung-Yeh&rft.date=2015-09-01&rft.eissn=1365-2958&rft.volume=97&rft.issue=6&rft.spage=1128&rft.epage=1141&rft_id=info:doi/10.1111%2Fmmi.13089&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0950-382X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0950-382X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0950-382X&client=summon |