Correlation between PARP-1 Val762Ala polymorphism and the risk of lung cancer in a Chinese population

• Published in: Tumor biology Vol. 36; no. 1; p. 177
• Main Authors: Yu, Ping, Liu, Yun-Peng, Zhang, Jing-Dong, Qu, Xiu-Juan, Jin, Bo, Zhang, Ye
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.01.2015
• Subjects: Adenocarcinoma - genetics; Adult; Amino Acid Substitution; Carcinoma, Squamous Cell - genetics; Case-Control Studies; China; Correlation analysis; Female; Genetic Association Studies; Genetic Predisposition to Disease; Health risk assessment; Humans; Lung cancer; Lung Neoplasms - genetics; Male; Middle Aged; Poly (ADP-Ribose) Polymerase-1; Poly(ADP-ribose) Polymerases - genetics; Polymorphism; Polymorphism, Single Nucleotide; Population genetics; Sequence Analysis, DNA; Small Cell Lung Carcinoma - genetics; China
• DOI: 10.1007/s13277-014-2373-3
• ISSN: 1010-4283; 1423-0380

The aim of this study was to investigate the relationship of the PARP-1 Val762Ala (rs1136410 T>C) polymorphism and the risk of lung cancer. A population-based case-control study of 373 lung cancer patients and 360 healthy control subjects (individually matched on age and gender) in a Chinese population was conducted. Genomic DNA was extracted by the phenol-chloroform method from the peripheral blood. PARP-1 Val762Ala polymorphism was identified using polymerase chain reaction-restriction fragments length polymorphism technique. After adjusting for age, tobacco smoking, gender, smoking index, and drinking status, logistic regression analysis demonstrated that CC genotype in PARP-1 Val762Ala polymorphism had an increased risk of lung cancer compared with TT genotype (OR = 1.59, 95 % CI = 1.03 ~ 2.50, P = 0.048), a statistically difference that still existed when merging CC and TC genotypes (OR = 1.56, 95 % CI = 1.03 ~ 2.44, P = 0.042). However, no obvious difference was found between TT and TC (OR = 1.54, 95 % CI = 0.96 ~ 2.44, P = 0.073). Subgroup analysis by histological type indicated that adenocarcinoma patients had higher frequencies of CC or TC+CC genotypes than healthy controls (CC: OR = 1.85, 95 % CI = 1.12 ~ 3.03, P = 0.015; TC+CC: OR = 1.67, 95 % CI = 1.06 ~ 2.63, P = 0.027, respectively), but no statistically significant difference within each genotype in squamous cell carcinoma or small cell lung cancer (all P > 0.05). Our findings support the view that PARP-1 Val762Ala polymorphism may contribute to an increased risk of lung cancer in the Chinese population, especially for adenocarcinoma.