MnO2 nanosheets as a carrier and accelerator for improved live-cell biosensing application of CRISPR/Cas12a

• Published in: Chemical science (Cambridge) Vol. 13; no. 15; pp. 4364 - 4371
• Main Authors: Dong-Xia, Wang, Ya-Xin, Wang, Wang, Jing, Jia-Yi, Ma, Liu, Bo, An-Na, Tang, De-Ming, Kong
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 13.04.2022; The Royal Society of Chemistry
• Subjects: Cells (biology); Chemistry; CRISPR; Genetic modification; Glutathione; Manganese dioxide; Nanosheets
• ISSN: 2041-6520; 2041-6539
• DOI: 10.1039/d1sc06383a

Besides gene-editing, the CRISPR/Cas12a system has also been widely used in in vitro biosensing, but its applications in live-cell biosensing are rare. One reason is lacking appropriate carriers to synchronously deliver all components of the CRISPR/Cas12a system into living cells. Herein, we demonst...