Desensitization and resensitization of δ‐opioid receptor‐mediated Ca2+ channel inhibition in NG108‐15 cells
1 To approach the mechanisms underlying desensitization of the opioid receptor‐mediated Ca2+ channel inhibition, the effects of prolonged application of [D‐Ala2, D‐Leu5]enkephalin (DADLE) on Ba2+ currents (IBa) through Ca2+ channels were analysed in NG108‐15 neuroblastoma × glioma hybrid cells. 2 In...
Saved in:
Published in | British journal of pharmacology Vol. 123; no. 6; pp. 1111 - 1118 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.03.1998
Nature Publishing |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | 1
To approach the mechanisms underlying desensitization of the opioid receptor‐mediated Ca2+ channel inhibition, the effects of prolonged application of [D‐Ala2, D‐Leu5]enkephalin (DADLE) on Ba2+ currents (IBa) through Ca2+ channels were analysed in NG108‐15 neuroblastoma × glioma hybrid cells.
2
Inhibition of IBa by 100 nM DADLE desensitized by 57% with a time constant of 4.4 min.
3
Maximal desensitization of the δ‐opioid receptor‐Ca2+ channel coupling was attained by 1 μM DADLE. The EC50 value for desensitization was estimated to be 78 nM.
4
RNA blot hybridization analysis and immunoblot analysis revealed the expression of β‐adrenoceptor kinase‐1 (βARK1) in NG108‐15 cells.
5
Heparin, an inhibitor of βARK, significantly reduced the magnitude and rate of desensitization, whereas Rp‐cyclic AMPS and PKI (14‐24)amide, inhibitors of cyclic AMP‐dependent protein kinase (PKA), or long‐term treatment with phorbol 12‐myristate 13‐acetate to induce down‐regulation of protein kinase C (PKC) had no significant effect.
6
Recovery from desensitization (resensitization) proceeded with a time constant of 6.7 min. Okadaic acid, an inhibitor of serine/threonine phosphatases 1 and 2A, significantly attenuated the degree of resensitization.
7
In summary, we have characterized the time course and concentration‐dependence of the desensitization of DADLE‐induced IBa inhibition in NG108‐15 cells. This desensitization was reversible after removal of DADLE. It is suggested that βARK, but neither PKA nor PKC, is involved in desensitization, while serine/threonine phosphatases mediate resensitization.
British Journal of Pharmacology (1998) 123, 1111–1118; doi:10.1038/sj.bjp.0701733 |
---|---|
AbstractList | 1. To approach the mechanisms underlying desensitization of the opioid receptor-mediated Ca2+ channel inhibition, the effects of prolonged application of [D-Ala2, D-Leu5]enkephalin (DADLE) on Ba2+ currents (I(Ba)) through Ca2+ channels were analysed in NG108-15 neuroblastoma x glioma hybrid cells. 2. Inhibition of I(Ba) by 100 nM DADLE desensitized by 57% with a time constant of 4.4 min. 3. Maximal desensitization of the delta-opioid receptor-Ca2+ channel coupling was attained by 1 microM DADLE. The EC50 value for desensitization was estimated to be 78 nM. 4. RNA blot hybridization analysis and immunoblot analysis revealed the expression of beta-adrenoceptor kinase-1 (betaARK1) in NG108-15 cells. 5. Heparin, an inhibitor of betaARK, significantly reduced the magnitude and rate of desensitization, whereas Rp-cyclic AMPS and PKI (14-24)amide, inhibitors of cyclic AMP-dependent protein kinase (PKA), or long-term treatment with phorbol 12-myristate 13-acetate to induce down-regulation of protein kinase C (PKC) had no significant effect. 6. Recovery from desensitization (resensitization) proceeded with a time constant of 6.7 min. Okadaic acid, an inhibitor of serine/threonine phosphatases 1 and 2A, significantly attenuated the degree of resensitization. 7. In summary, we have characterized the time course and concentration-dependence of the desensitization of DADLE-induced I(Ba) inhibition in NG108-15 cells. This desensitization was reversible after removal of DADLE. It is suggested that betaARK, but neither PKA nor PKC, is involved in desensitization, while serine/threonine phosphatases mediate resensitization. 1 To approach the mechanisms underlying desensitization of the opioid receptor‐mediated Ca2+ channel inhibition, the effects of prolonged application of [D‐Ala2, D‐Leu5]enkephalin (DADLE) on Ba2+ currents (IBa) through Ca2+ channels were analysed in NG108‐15 neuroblastoma × glioma hybrid cells. 2 Inhibition of IBa by 100 nM DADLE desensitized by 57% with a time constant of 4.4 min. 3 Maximal desensitization of the δ‐opioid receptor‐Ca2+ channel coupling was attained by 1 μM DADLE. The EC50 value for desensitization was estimated to be 78 nM. 4 RNA blot hybridization analysis and immunoblot analysis revealed the expression of β‐adrenoceptor kinase‐1 (βARK1) in NG108‐15 cells. 5 Heparin, an inhibitor of βARK, significantly reduced the magnitude and rate of desensitization, whereas Rp‐cyclic AMPS and PKI (14‐24)amide, inhibitors of cyclic AMP‐dependent protein kinase (PKA), or long‐term treatment with phorbol 12‐myristate 13‐acetate to induce down‐regulation of protein kinase C (PKC) had no significant effect. 6 Recovery from desensitization (resensitization) proceeded with a time constant of 6.7 min. Okadaic acid, an inhibitor of serine/threonine phosphatases 1 and 2A, significantly attenuated the degree of resensitization. 7 In summary, we have characterized the time course and concentration‐dependence of the desensitization of DADLE‐induced IBa inhibition in NG108‐15 cells. This desensitization was reversible after removal of DADLE. It is suggested that βARK, but neither PKA nor PKC, is involved in desensitization, while serine/threonine phosphatases mediate resensitization. British Journal of Pharmacology (1998) 123, 1111–1118; doi:10.1038/sj.bjp.0701733 To approach the mechanisms underlying desensitization of the opioid receptor-mediated Ca 2+ channel inhibition, the effects of prolonged application of [ D -Ala 2 , D -Leu 5 ]enkephalin (DADLE) on Ba 2+ currents ( I Ba ) through Ca 2+ channels were analysed in NG108-15 neuroblastoma × glioma hybrid cells. Inhibition of I Ba by 100 n M DADLE desensitized by 57% with a time constant of 4.4 min. Maximal desensitization of the δ-opioid receptor-Ca 2+ channel coupling was attained by 1 μ M DADLE. The EC 50 value for desensitization was estimated to be 78 n M . RNA blot hybridization analysis and immunoblot analysis revealed the expression of β-adrenoceptor kinase-1 (βARK1) in NG108-15 cells. Heparin, an inhibitor of βARK, significantly reduced the magnitude and rate of desensitization, whereas Rp-cyclic AMPS and PKI (14-24)amide, inhibitors of cyclic AMP-dependent protein kinase (PKA), or long-term treatment with phorbol 12-myristate 13-acetate to induce down-regulation of protein kinase C (PKC) had no significant effect. Recovery from desensitization (resensitization) proceeded with a time constant of 6.7 min. Okadaic acid, an inhibitor of serine/threonine phosphatases 1 and 2A, significantly attenuated the degree of resensitization. In summary, we have characterized the time course and concentration-dependence of the desensitization of DADLE-induced I Ba inhibition in NG108-15 cells. This desensitization was reversible after removal of DADLE. It is suggested that βARK, but neither PKA nor PKC, is involved in desensitization, while serine/threonine phosphatases mediate resensitization. |
Author | Mima, Hiroyuki Kato, Shigehisa Morikawa, Hitoshi Shoda, Takehiro Fukuda, Kazuhiko Mori, Kenjiro |
AuthorAffiliation | Department of Anesthesia, Kyoto University Hospital, Kyoto 606-01, Japan |
AuthorAffiliation_xml | – name: Department of Anesthesia, Kyoto University Hospital, Kyoto 606-01, Japan |
Author_xml | – sequence: 1 givenname: Hitoshi surname: Morikawa fullname: Morikawa, Hitoshi – sequence: 2 givenname: Kazuhiko surname: Fukuda fullname: Fukuda, Kazuhiko – sequence: 3 givenname: Hiroyuki surname: Mima fullname: Mima, Hiroyuki – sequence: 4 givenname: Takehiro surname: Shoda fullname: Shoda, Takehiro – sequence: 5 givenname: Shigehisa surname: Kato fullname: Kato, Shigehisa – sequence: 6 givenname: Kenjiro surname: Mori fullname: Mori, Kenjiro |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2197978$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/9559894$$D View this record in MEDLINE/PubMed |
BookMark | eNpdkcFu1DAQhi1UVLaFKzekHBAXlO1MbMf2BQkW2iJVhQOcrUnWy3qVtUOcBZUTj8DD8Bx9CJ4EL41WwGmk-f75Z-z_hB2FGBxjjxHmCFyfpc282fRzUICK83tshkLVpeQaj9gMAFSJqPUDdpLSBiBDJY_ZsZHSaCNmbHjtkgvJj_4bjT6GgsKyGP7rxVVx-_PX9x-x99Hvcev6MQ65s3VLT6NbFguqnhftmkJwXeHD2jf-z6gPxfUFgs5alEXrui49ZPdX1CX3aKqn7OP5mw-Ly_Lq3cXbxcursudQq9IJA1JJ2YiWVF070FgLQVoTcVeBXgEBlyjbRi5FDQJRVVUlGoccNdScn7IXd779rsl3ti6MA3W2H_yWhhsbydt_SfBr-yl-sShrWWnIBs8mgyF-3rk02q1P-ydQcHGXrDIaDDcyC5_8vemwYvrlzJ9OnFJL3Wqg0Pp0kFVolFE6y_id7Kvv3M0BI9h90jZtbE7aTknbV-8vpQHFfwOi46HF |
CODEN | BJPCBM |
ContentType | Journal Article |
Copyright | 1998 British Pharmacological Society 1998 INIST-CNRS Copyright 1998, Nature Publishing Group 1998 Nature Publishing Group |
Copyright_xml | – notice: 1998 British Pharmacological Society – notice: 1998 INIST-CNRS – notice: Copyright 1998, Nature Publishing Group 1998 Nature Publishing Group |
DBID | IQODW CGR CUY CVF ECM EIF NPM 7X8 5PM |
DOI | 10.1038/sj.bjp.0701733 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Pharmacy, Therapeutics, & Pharmacology |
EISSN | 1476-5381 |
EndPage | 1118 |
ExternalDocumentID | 9559894 2197978 BPH5907 |
Genre | article Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- .3N .55 .GJ 05W 0R~ 1OC 23N 24P 2WC 31~ 33P 36B 3O- 3SF 3V. 4.4 52U 52V 53G 5GY 6J9 7RV 7X7 8-0 8-1 88E 8AO 8FE 8FH 8FI 8FJ 8R4 8R5 8UM A00 AAESR AAEVG AAHHS AANLZ AAONW AASGY AAXRX AAZKR ABCUV ABDBF ABPVW ABQWH ABUWG ABXGK ACAHQ ACCFJ ACCZN ACFBH ACGFO ACGFS ACGOF ACMXC ACPOU ACPRK ACXBN ACXQS ADBBV ADBTR ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN ADZOD AEEZP AEGXH AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFFPM AFGKR AFKRA AFPWT AFRAH AFZJQ AHBTC AHMBA AIACR AIAGR AITYG AIURR AIWBW AJBDE ALAGY ALIPV ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB AOIJS ATUGU AZBYB AZVAB B0M BAFTC BAWUL BBNVY BENPR BFHJK BHBCM BHPHI BKEYQ BMXJE BPHCQ BRXPI BVXVI C45 CAG CCPQU COF CS3 DCZOG DIK DRFUL DRMAN DRSTM DU5 E3Z EAD EAP EAS EBC EBD EBS ECV EJD EMB EMK EMOBN ENC ESX EX3 F5P FUBAC FYUFA G-S GODZA GX1 H.X HCIFZ HGLYW HMCUK HYE HZ~ J5H KBYEO LATKE LEEKS LH4 LITHE LK8 LOXES LSO LUTES LW6 LYRES M1P M7P MEWTI MK0 MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM MY~ N9A NAPCQ NF~ O66 O9- OIG OK1 OVD P2P P2W P4E PQQKQ PROAC PSQYO Q.N Q2X QB0 RIG ROL RPM RWI SJN SUPJJ SV3 TEORI TR2 TUS UKHRP UPT WBKPD WH7 WHWMO WIH WIJ WIK WIN WOHZO WOW WVDHM WXSBR X7M XV2 Y6R YHG ZGI ZXP ZZTAW ~8M ~S- 08R AAJUZ AAPBV AAUGY AAVGM ABCVL ABHUG ABPTK ABWRO ACXME ADAWD ADDAD AFVGU AGJLS IQODW ZA5 CGR CUY CVF ECM EIF NPM 7X8 5PM |
ID | FETCH-LOGICAL-p3067-e4905755b4ca766e081644a88aa3e208f0a03515cb5d46041172224be13180633 |
IEDL.DBID | RPM |
ISSN | 0007-1188 |
IngestDate | Tue Sep 17 21:27:34 EDT 2024 Fri Oct 25 10:17:22 EDT 2024 Sat Sep 28 07:40:32 EDT 2024 Sun Oct 29 17:08:42 EDT 2023 Sat Aug 24 00:43:43 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Keywords | Cell culture Phosphoprotein phosphatase Enkephalin Phosphoric monoester hydrolases Electrophysiology Patch clamp method Opiates Esterases Ionic channel Neuroblastoma δ Opioid receptor Dose activity relation β-Adrenergic receptor Malignant glioma Established cell line Sensitization Tumor cell Calcium Cations Nervous system diseases Desensitization Enzyme Transferases Malignant tumor In vitro Protein kinase Central nervous system disease Hydrolases |
Language | English |
License | CC BY 4.0 |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-p3067-e4905755b4ca766e081644a88aa3e208f0a03515cb5d46041172224be13180633 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1038/sj.bjp.0701733 |
PMID | 9559894 |
PQID | 79809395 |
PQPubID | 23479 |
PageCount | 8 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_1565280 proquest_miscellaneous_79809395 pubmed_primary_9559894 pascalfrancis_primary_2197978 wiley_primary_10_1038_sj_bjp_0701733_BPH5907 |
PublicationCentury | 1900 |
PublicationDate | March 1998 |
PublicationDateYYYYMMDD | 1998-03-01 |
PublicationDate_xml | – month: 03 year: 1998 text: March 1998 |
PublicationDecade | 1990 |
PublicationPlace | Oxford, UK |
PublicationPlace_xml | – name: Oxford, UK – name: Basingstoke – name: England |
PublicationTitle | British journal of pharmacology |
PublicationTitleAlternate | Br J Pharmacol |
PublicationYear | 1998 |
Publisher | Blackwell Publishing Ltd Nature Publishing |
Publisher_xml | – name: Blackwell Publishing Ltd – name: Nature Publishing |
References | 1993; 327 1987; 31 1987; 32 1990; 34 1996; 17 1989; 1 1994; 270 1995; 15 1991; 11 1991; 97 1992 1996; 16 1992; 12 1990; 265 1989; 409 1992; 451 1995; 48 1991; 88 1993; 11 1989; 264 1992; 258 1982; 22 1995; 65 1988; 256 1988; 251 1992; 42 1994; 72 1996; 67 1996; 117 1988; 335 |
References_xml | – volume: 11 start-page: 995 year: 1993 end-page: 1006 article-title: Drug addiction: A model for the molecular basis of neural plasticity publication-title: Neuron – volume: 15 start-page: 7485 year: 1995 end-page: 7499 article-title: Protein kinase C involvement in homologous desensitization of the β‐opioid receptor coupled to G ‐phospholipase C activation in oocytes publication-title: J. Neurosci. – volume: 12 start-page: 4045 year: 1992 end-page: 4055 article-title: The G‐protein‐coupled receptor kinases βARK1 and βARK2 are widely distributed at synapses in rat brain publication-title: J. Neurosci. – volume: 72 start-page: 700 year: 1994 end-page: 708 article-title: Intracerebral methionine‐enkephalin, serum Cortisol, and serum β‐endorphin during acute exposure of sheep to physical or isolation stress publication-title: J. Animal Sci. – volume: 32 start-page: 633 year: 1987 end-page: 638 article-title: Cellular mechanisms of opioid tolerance: studies in single brain neurons publication-title: Mol. Pharmacol. – volume: 22 start-page: 1 year: 1982 end-page: 4 article-title: Loss of opiate receptor activity in neuroblastoma × glioma Ng108–15 hybrid cells after chronic opiate treatment: a multiple‐step process publication-title: Mol. Pharmacol. – volume: 256 start-page: 283 year: 1988 end-page: 290 article-title: Inhibitory effect of a marine‐sponge toxin, okadaic acid, on protein phosphatases: specificity and kinetics publication-title: Biochem. J. – volume: 65 start-page: 1403 year: 1995 end-page: 1406 article-title: Coupling of the cloned μ‐opioid receptor with the ω‐conotoxin‐sensitive Ca current in Ng108–15 cells publication-title: J. Neurochem. – volume: 16 start-page: 579 year: 1996 end-page: 585 article-title: G protein‐coupled receptor kinase mediates desensitization of norepinephrine‐induced Ca channel inhibition publication-title: Neuron – volume: 11 start-page: 2574 year: 1991 end-page: 2581 article-title: Transient homologous μ‐opioid receptor desensitization in rat locus coeruleus neurons publication-title: J. Neurosci. – volume: 15 start-page: 2995 year: 1995 end-page: 3012 article-title: Pharmacological dissection of multiple types of Ca channel currents in rat cerebellar granule neurons publication-title: J. Neurosci. – volume: 67 start-page: 1309 year: 1996 end-page: 1316 article-title: Functional coupling of the δ‐, μ‐, and κ‐opioid receptors to mitogen‐activated protein kinase and arachidonate release in Chinese hamster ovary cells publication-title: J. Neurochem. – start-page: 119 year: 1992 end-page: 147 – volume: 451 start-page: 229 year: 1992 end-page: 246 article-title: Calcium current modulation in frog sympathetic neurones: multiple neurotransmitters and G proteins publication-title: J. Physiol. – volume: 48 start-page: 173 year: 1995 end-page: 177 article-title: Agonist‐dependent phosphorylation of the mouse δ‐opioid receptor: involvement of G protein‐coupled receptor kinases but not protein kinase C publication-title: Mol. Pharmacol. – volume: 1 start-page: 141 year: 1989 end-page: 147 article-title: Noradrenaline‐ and enkephalin‐induced inhibition of voltage‐sensitive calcium currents in Ng108–15 hybrid cells: Transduction mechanisms publication-title: Eur. J. Neurosci. – volume: 251 start-page: 757 year: 1988 end-page: 762 article-title: A mechanistic and kinetic analysis of the interactions of the diastereoisomers of adenosine 3′, 5′‐(cyclic)phosphorothioate with purified cyclic Amp‐dependent protein kinase publication-title: Biochem. J. – volume: 16 start-page: 1479 year: 1996 end-page: 1485 article-title: Opioid desensitization: interactions with G‐protein‐coupled receptors in the locus coeruleus publication-title: J. Neurosci. – volume: 17 start-page: 264 year: 1996 end-page: 269 article-title: The stimulatory effects of opioids and their possible role in the development of tolerance publication-title: Trends Pharmacol. Sci. – volume: 264 start-page: 8802 year: 1989 end-page: 8810 article-title: Primary structural determinants essential for potent inhibition of cAMP‐dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat‐stable inhibitor protein publication-title: J. Biol. Chem. – volume: 409 start-page: 221 year: 1989 end-page: 240 article-title: Somatostatin blocks a calcium current in rat sympathetic ganglion neurones publication-title: J. Physiol. – volume: 117 start-page: 161 year: 1996 end-page: 169 article-title: Differential desensitization of μ‐ and δ‐opioid receptors in selected neural pathways following chronic morphine treatment publication-title: Br. J. Pharmacol. – volume: 31 start-page: 159 year: 1987 end-page: 168 article-title: Gtpase and adenylate cyclase desensitize at different rates in Ng108–15 cells publication-title: Mol. Pharmacol. – volume: 88 start-page: 8855 year: 1991 end-page: 8859 article-title: Tonic inhibition and rebound facilitation of a neuronal calcium channel by a Gtp‐binding protein publication-title: Proc. Natl. Acad. Sci. U.S.A. – volume: 34 start-page: 35 year: 1990 end-page: 46 article-title: Microdialysis measurement of in vivo neuropeptide release publication-title: J. Neurosci. Methods – volume: 270 start-page: 466 year: 1994 end-page: 474 article-title: Opioid inhibition and desensitization of calcium channel currents in rat dorsal root ganglion neurons publication-title: J. Pharmacol. Exp. Ther. – volume: 335 start-page: 355 year: 1988 end-page: 358 article-title: Selective coupling with K currents of muscarinic acetylcholine receptor subtypes in Ng108–15 cells publication-title: Nature – volume: 270 start-page: 1381 year: 1994 end-page: 1386 article-title: Molecular mechanisms of agonist‐induced desensitization of the cloned mouse opioid receptor publication-title: J. Pharmacol. Exp. Ther. – volume: 97 start-page: 521 year: 1991 end-page: 539 article-title: Sodium currents during differentiation in a human neuroblastoma cell line publication-title: J. Gen. Physiol. – volume: 258 start-page: 1952 year: 1992 end-page: 1955 article-title: Cloning of a delta opioid receptor by functional expression publication-title: Science – volume: 327 start-page: 311 year: 1993 end-page: 314 article-title: Primary structures and expression from cDNAs of rat opioid receptor δ‐ and μ‐subtypes publication-title: FEES Lett. – volume: 42 start-page: 656 year: 1992 end-page: 665 article-title: Protein kinase C activation increases the rate and magnitude of agonist‐induced δ‐opioid receptor down‐regulation in Ng108–15 cells publication-title: Mol Pharmacol. – volume: 15 start-page: 2396 year: 1995 end-page: 2406 article-title: The human μ opioid receptor: modulation of functional desensitization by calcium/calmodulin‐dependent protein kinase and protein kinase C publication-title: J. Neurosci. – volume: 265 start-page: 3202 year: 1990 end-page: 3209 article-title: Multiple pathways of rapid β ‐adrenergic receptor desensitization: delineation with specific inhibitors publication-title: J. Biol. Chem. |
SSID | ssj0014775 |
Score | 1.6902189 |
Snippet | 1
To approach the mechanisms underlying desensitization of the opioid receptor‐mediated Ca2+ channel inhibition, the effects of prolonged application of... 1. To approach the mechanisms underlying desensitization of the opioid receptor-mediated Ca2+ channel inhibition, the effects of prolonged application of... To approach the mechanisms underlying desensitization of the opioid receptor-mediated Ca 2+ channel inhibition, the effects of prolonged application of [ D... |
SourceID | pubmedcentral proquest pubmed pascalfrancis wiley |
SourceType | Open Access Repository Aggregation Database Index Database Publisher |
StartPage | 1111 |
SubjectTerms | Animals beta-Adrenergic Receptor Kinases Biological and medical sciences Calcium Channel Blockers - pharmacology calcium channels COS Cells Cyclic AMP-Dependent Protein Kinases - genetics Cyclic AMP-Dependent Protein Kinases - metabolism desensitization Dose-Response Relationship, Drug Enkephalin, Leucine-2-Alanine - pharmacology Hybrid Cells Medical sciences Neuropharmacology Neurotransmitters. Neurotransmission. Receptors NG108‐15 cells opioid δ receptors Opioids patch clamp Peptidergic system (neuropeptide, opioid peptide, opiates...). Adenosinergic and purinergic systems Pharmacology. Drug treatments Protein Kinase C - metabolism Receptors, Opioid, delta - drug effects Receptors, Opioid, delta - physiology resensitization Tumor Cells, Cultured β‐adrenoceptor kinases |
Title | Desensitization and resensitization of δ‐opioid receptor‐mediated Ca2+ channel inhibition in NG108‐15 cells |
URI | https://onlinelibrary.wiley.com/doi/abs/10.1038%2Fsj.bjp.0701733 https://www.ncbi.nlm.nih.gov/pubmed/9559894 https://search.proquest.com/docview/79809395 https://pubmed.ncbi.nlm.nih.gov/PMC1565280 |
Volume | 123 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Na9wwEB2ygUKglH6Fum1SHUouXe_almTJx3bbZCkk7CGBvRnJlomXjW3WySH_q78jvykj2d502556MxZCQjNIb5h5bwA-59qERS6oj2A09hkzoa-kEj5PlC4yg4GztkTh84t4fsV-LvlyD_jAhXFF-5kuJ9X6ZlKV1662srnJpkOd2HRxPsOYg0cymI5gJCgdQvQ-dcCE6NoWWPXDUMpBqZHKabua6FUzQS8Pcd4BPLPya9L2K37eqBbPpOiaWfwLbf5dNPk7mHWv0elLeNHDSPK12-4r2DPVazhZdDrU92Ny-USrasfkhCyeFKrv38D6u-Uc2WqtjoRJVJWTzR__6oI8_PLrpqxLO2jLX-qN75gmiFLJTEVfiKUNV2ZNyuq61K74Cz_JxVlo1Vw5sVmB9i1cnf64nM39vu2C39j4wTcssSCOa5YpEcfGtuZgTEmpFDVRIItA2fQjzzTPWRywEDEQAgE0Ot4PiHjoIexXdWXeAS6DACW2ej2CMpUIJVTGeZTkWRRzrmIPjnYOPm06iY0U71GB8a0HnwZDpOj2dteqMvVdm4pEBglNuAeHnVm2U3ubeiB27LUdt3rauyPoZk5Xu3crD8bOstsZLk9PZdquUnSftHef9NtizpNAvP_vhT7AwcBwDMKPsH-7uTNHCHFu9TGMzpbhsXPsR3cI_P0 |
link.rule.ids | 230,315,733,786,790,891,27946,27947,53816,53818 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9NAEB2VIkQlxHeFC6V7QL0QO3a8610fIVACNFEOKerN2rXXqkNqW3FyaH8Xv4PfxKw_UlK4wM3yarW2Z3b9RvPmDcCbRGkvTbhvIxgNbEq1Z0shuc1CqdJYY-CsTKHweBKMzuiXc3a-A6yrhalJ-7HKnHxx6eTZRc2tLC_jfscT60_HQ4w52EC4_Ttwl5nEVxekt8kDynnTuMDoH3pCdFqNvuhXc0fNSwf93OO-vwf3jACbMB2LH5Sywq-SNu0s_oY3_6RN_g5n6__RySP41r1JQ0P57qxXyomvb4k8_vOrPoaHLUIl75rhJ7Cj86dwPG0krq96ZHZTsVX1yDGZ3ohfXz2DxQdTzmSIYE19J5F5Qpa37hUp-fnDLsqsyMygYdYUS7suYkEATIZy8JaYiuRcL0iWX2Sq5pXhJZl88oxQLCMm4VA9h7OTj7PhyG47OtilCU1sTUODD5miseRBoE3XD0qlEFL6euCK1JUms8lixRIauNRDeIUYA_0Jjx4EU_4-7OZFrl8ALoPYJzBSQNynMuSSy5ixQZjEg4AxGVhwuGXRqGzUOyI8ojmGzhYcdRaOcEeZp5a5LtZVxEPhhn7ILNhv7L2Z2jqLBXzLETbjRqp7ewTNWkt2t2a0oFe7zGZGTQHwRVTNI_TLqPXL6P10xEKXH_z3QkdwfzQbn0annydfX8JeV0jpeq9gd7Vc60NEUiv1ut43vwA2tx4M |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BEagS4l0RoNQH1Aubtx07R9iyLI-ucmilikvkJI6aZZtEm91D-V38Dn4T4zy23cKptyiO5Tgzdr6Rv_kG4F2WKDfPuG8iGA1MSpVrSiG5yUKZ5KnCwDnRicLHs2B6Sr-esbNrpb5a0n6aFFa5uLDK4rzlVtYXqT3wxOzoeIwxB_OEY9dZbt-Fe0z_dYZAvT9AoJx3xQu0BqIrxKDX6Au7mVvJvLbQ113u-7twX4uwCV21-GEtG_wyeVfS4n-Y81_q5HVI2_6TJo_hxzCbjory01qvEiv9dUPo8VbTfQKPeqRKPnSPPIU7qnwGh1EndX05IidXmVvNiByS6EoE-_I5LI50WpMmhHV5nkSWGVneuFfl5M9vs6qLqtCNmmFTLc02mQWBMBlL7z3RmcmlWpCiPC-Sll-Gl2T22dWCsYzog4fmBZxOPp2Mp2Zf2cGsdYhiKhpqnMgSmkoeBEpX_6BUCiGlrzxH5I7UJ5wsTVhGA4e6CLMQa6Bf4RaEoMrfg52yKtVLwGEQAwVaEoj7VIZccpky5oVZ6gWMycCA_S2rxnWn4hHjVs0xhDbgYLByjCtLv7UsVbVuYh4KJ_RDZsBeZ_NN195hDOBbzrBp15Ld2y1o2la6uzelAaPWbTY9WiqAL-JmHqNvxr1vxh-jKQsd_urWAx3Ag-hoEn__Mvv2GnaHfErHfQM7q-Va7SOgWiVv26XzFymRIIw |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Desensitization+and+resensitization+of+%CE%B4%E2%80%90opioid+receptor%E2%80%90mediated+Ca2%2B+channel+inhibition+in+NG108%E2%80%9015+cells&rft.jtitle=British+journal+of+pharmacology&rft.au=Morikawa%2C+Hitoshi&rft.au=Fukuda%2C+Kazuhiko&rft.au=Mima%2C+Hiroyuki&rft.au=Shoda%2C+Takehiro&rft.date=1998-03-01&rft.pub=Blackwell+Publishing+Ltd&rft.issn=0007-1188&rft.eissn=1476-5381&rft.volume=123&rft.issue=6&rft.spage=1111&rft.epage=1118&rft_id=info:doi/10.1038%2Fsj.bjp.0701733&rft.externalDBID=10.1038%252Fsj.bjp.0701733&rft.externalDocID=BPH5907 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0007-1188&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0007-1188&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0007-1188&client=summon |