An episomal mammalian replicon: sequence‐independent binding of the origin recognition complex

• Published in: The EMBO journal Vol. 23; no. 1; pp. 191 - 201
• Main Authors: Schaarschmidt, Daniel, Baltin, Jens, Stehle, Isa M, Lipps, Hans J, Knippers, Rolf
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 14.01.2004; Blackwell Publishing Ltd
• Subjects: Animals; Base Pairing; Base Sequence; Cell Cycle Proteins - metabolism; CHO Cells; Chromatin - metabolism; Clone Cells; Cricetinae; Cricetulus; Deoxyribonucleic acid; DNA; DNA, Circular - chemistry; DNA, Circular - metabolism; DNA-Binding Proteins - metabolism; HeLa Cells; Humans; initiation of DNA replication; mammalian episome; Mammals; Mammals - genetics; Matrix Attachment Regions; Mcm3 protein; Minichromosome Maintenance Complex Component 3; Nuclear Proteins; Orc1 protein; Orc2 protein; origin of replication; Origin Recognition Complex; Plasmids; Polymerase Chain Reaction; Precipitin Tests; Promoter Regions, Genetic; Replicon

An extrachromosomally replicating plasmid was used to investigate the specificity by which the origin recognition complex (ORC) interacts with DNA sequences in mammalian cells in vivo. We first showed that the plasmid pEPI‐1 replicates semiconservatively in a once‐per‐cell‐cycle manner and is stably...