Effects of conformational selectivity and of overlapping kinetically influential ionizations on the characteristics of pH-dependent enzyme kinetics. Implications of free-enzyme pKa variability in reactions of papain for its catalytic mechanism

• Published in: Biochemical journal Vol. 211; no. 3; pp. 701 - 708
• Main Authors: Brocklehurst, K, Willenbrock, S J, Salih, E
• Format: Journal Article
• Language: English
• Published: England 01.06.1983
• Subjects: Hydrogen-Ion Concentration; Kinetics; Mathematics; Models, Chemical; Papain - metabolism; Protein Conformation; Protons

The effects of selection by a small molecule, when binding to a protein, of a particular conformation from an equilibrium stereopopulation on the characteristics of the pH-dependence of reaction with a reactivity probe or substrate were determined by analysis of an appropriate kinetic model. For rea...