Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol

• Published in: Nature chemical biology Vol. 13; no. 3; pp. 268 - 274
• Main Authors: Liu, Shu-Lin, Sheng, Ren, Jung, Jae Hun, Wang, Li, Stec, Ewa, O'Connor, Matthew J, Song, Seohyoen, Bikkavilli, Rama Kamesh, Winn, Robert A, Lee, Daesung, Baek, Kwanghee, Ueda, Kazumitsu, Levitan, Irena, Kim, Kwang-Pyo, Cho, Wonhwa
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2017; Nature Publishing Group
• Subjects: 631/80/86; 631/92/287; 631/92/314; 631/92/96; Asymmetry; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Cell Biology; Cell Line; Cell Membrane - chemistry; Chemistry; Chemistry/Food Science; Cholesterol; Cholesterol - analysis; HEK293 Cells; Homeostasis; Humans; Hyperlipidemia; Lipids; Lipids - chemistry; Membranes; Sensors; United States > US; Illinois; Chicago Illinois

Orthogonal cholesterol sensors that are useful for imaging cholesterol in the plasma membrane leaflets reveal an asymmetry in which a high outer leaflet concentration is important for signaling processes and is potentially actively maintained. Controlled distribution of lipids across various cell me...