Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7

• Published in: Molecular vision Vol. 23; pp. 228 - 241
• Main Authors: Zhang, Youwen, Cross, Samuel D, Stanton, James B, Marmorstein, Alan D, Le, Yun Zheng, Marmorstein, Lihua Y
• Format: Journal Article
• Language: English
• Published: United States Molecular Vision 14.04.2017
• Subjects: Advanced glycosylation end products; Age; Alleles; Animals; Atrophy; Autophagy; Autophagy-Related Protein 5 - genetics; Autophagy-Related Protein 7 - genetics; Biomarkers - metabolism; Cre recombinase; Defects; Deoxyguanosine; Deoxyribonucleic acid; DNA; Doxycycline; Electron microscopy; Electroretinography; Female; Fluorescent Antibody Technique, Indirect; Gene Deletion; Glycosylation; Hypertrophy; Immunofluorescence; Lipids; Macular degeneration; Macular Degeneration - genetics; Macular Degeneration - pathology; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Nitrotyrosine; Phagocytosis; Phenotypes; Retina; Retinal degeneration; Retinal Degeneration - genetics; Retinal Degeneration - pathology; Retinal Pigment Epithelium - metabolism; Retinal Pigment Epithelium - pathology; Reverse Transcriptase Polymerase Chain Reaction; Rodents; Transgenic mice; Vacuoles; Vascularization

To examine the effects of autophagy deficiency induced by RPE-specific deletion of or in mice as a function of age. Conditional knockout mice with a floxed allele of or were crossed with inducible transgenic mice. -directed RPE-specific Cre recombinase expression was induced with doxycycline feeding...