Small-molecule–induced DNA damage identifies alternative DNA structures in human genes

• Published in: Nature chemical biology Vol. 8; no. 3; pp. 301 - 310
• Main Authors: Rodriguez, Raphaël, Miller, Kyle M, Forment, Josep V, Bradshaw, Charles R, Nikan, Mehran, Britton, Sébastien, Oelschlaegel, Tobias, Xhemalce, Blerta, Balasubramanian, Shankar, Jackson, Stephen P
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2012; Nature Publishing Group
• Subjects: 631/337/1427; 631/92/555; 631/92/613; 692/699/67; Aminoquinolines - chemical synthesis; Aminoquinolines - chemistry; Aminoquinolines - pharmacology; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cell Biology; Cell Cycle - drug effects; Cell Proliferation - drug effects; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chromatin; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - drug effects; DNA - genetics; DNA Damage; DNA structure; Drug Screening Assays, Antitumor; G-Quadruplexes - drug effects; Genetics; Genomes; genomics; Humans; Immunoprecipitation; Mammalian cells; Mammals; Molecular biology; Molecular Weight; Motility; Nucleotide sequence; Oncology; Pharmacology; Picolinic Acids - chemical synthesis; Picolinic Acids - chemistry; Picolinic Acids - pharmacology; Proto-Oncogene Mas; Proto-oncogenes; Src protein; Structure-Activity Relationship; Transcription; Tumor Cells, Cultured

Identifying DNA sequences that adopt alternative structures within the context of genomic DNA presents a major challenge. Pyridostatin, a G-quadruplex–specific chemical probe, was shown to induce DNA damage at specific genomic sites, including the proto-oncogene SRC , leading to cell cycle arrest in...