Chemical characteristics and cancer risk assessment of smokeless tobacco used in Tunisia (neffa)

• Published in: The Pan African medical journal Vol. 40; p. 45
• Main Authors: Guezguez, Fatma, Abdelwaheb, Mohamed, Anane, Ichraf, Rekik, Saleheddine, Saguem, Saad, Charfeddine, Bassem, Rouatbi, Sonia
• Format: Journal Article
• Language: English
• Published: Uganda African Field Epidemiology Network 2021; The African Field Epidemiology Network
• Subjects: Cadmium; Cadmium - analysis; Cancer; Carcinogens - analysis; Carcinogens - isolation & purification; Chromatography; Chromatography, High Pressure Liquid; Heavy metals; Humans; Lead - analysis; Neoplasms - etiology; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - isolation & purification; Risk Assessment; Spectrophotometry, Atomic; Tobacco; Tobacco Use - adverse effects; Tobacco, Smokeless - adverse effects; Tobacco, Smokeless - analysis; Toxicity; Tunisia; Tunisia; United States > US; risk assessment; carcinogens; polycyclic aromatic hydrocarbons; heavy metals; Smokeless tobacco
• ISSN: 1937-8688
• DOI: 10.11604/pamj.2021.40.45.24751

neffa, a form of air-dried smokeless tobacco used in North Africa, is spuriously perceived as a lower risk alternative to smoking. The objective of this study was to provide information on some harmful constituents of neffa and to use them for cancer risk assessment. a high-performance liquid chromatography method coupled with fluorescence detector was used to determine polycyclic aromatic hydrocarbons (PAHs) in one sample of neffa. An atomic absorption spectrometry was performed to determine the concentrations of lead and cadmium in three samples of neffa. The levels of toxicants found in neffa were used to assess for lifetime cancer risk as advocated by the US Environment Protection Agency. the determination of PAHs in neffa allowed the identification of phenanthrene and anthracene. However, the higher molecular weight PAHs such as Benzo(a)Pyrene B(a)P were not detected. The concentrations of cadmium and lead varied between 1.3 to 2.8µg/g and 1.7 to 4.6µg/g respectively. Cancer risk for cadmium and lead varied between 4.2E-03 to 9.3E-03 and 2.5E-06 to 6.4E-06 respectively. Cancer risk for Cd exceeded the range of 10E-04 to 10E-06 of an acceptable risk. neffa is not a healthy alternative for overcoming smoking addiction. It contains mineral and organic pulmonary toxicants. This study could serve as a scientific basis to inform consumers about the products´ toxicity and help them to quit smokeless tobacco (SLT) use.