Temperature-mediated processes in teleost immunity: binding and mitogenic properties of concanavalin A with channel catfish lymphocytes

• Published in: Immunology Vol. 58; no. 3; pp. 523 - 526
• Main Authors: BLY, J. E, CUCHENS, M. A, CLEM, L. W
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell 01.07.1986
• Subjects: Animals; Applied sciences; B-Lymphocytes - metabolism; Concanavalin A - metabolism; Concanavalin A - pharmacology; Dose-Response Relationship, Immunologic; Exact sciences and technology; Fishes - immunology; Kinetics; Lymphocyte Activation; Lymphocytes - immunology; Lymphocytes - metabolism; Other techniques and industries; T-Lymphocytes - metabolism; Temperature
• ISSN: 0019-2805; 1365-2567

Low (non-permissive) temperatures inhibit the proliferation responses of channel catfish T cells to stimulation with Con A. The study reported here was undertaken to ascertain if failure to bind Con A at non-permissive temperatures could explain the observed suppression at such temperatures. The influence of temperature on the binding of fluorescein-labelled succinyl Con A to channel catfish T and B cells was studied by cytofluorography. The results indicated that Con A bound equally well at both mitogenically permissive (22 degrees, 27 degrees and 32 degrees) and non-permissive (12 degrees and 17 degrees) temperatures. Hence, temperature effects on mitogen-binding to cell surface receptors cannot readily explain the observed suppression of channel catfish T-cell proliferative responses at non-permissive temperatures.