Heat stress‐induced B1 receptor synthesis in the rat: an ex vivo study

• Published in: British journal of pharmacology Vol. 125; no. 4; pp. 812 - 816
• Main Authors: Lagneux, Caroline, Innocenti‐Francillard, Patricia, Godin‐Ribuot, Diane, Bader, Mickael, Ribuot, Christophe
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.1998; Nature Publishing
• Subjects: [des‐Arg9]‐bradykinin; Animals; Aorta - metabolism; B1 receptor; Biological and medical sciences; Blood Pressure; Blotting, Southern; DNA, Complementary - chemical synthesis; Effects of various physical factors on living matter (vibrations, electric field, ultrasound, sound...); Endothelium, Vascular - drug effects; endotoxin; Fundamental and applied biological sciences. Psychology; Heat stress; Heat Stress Disorders - metabolism; In Vitro Techniques; Lipopolysaccharides - pharmacology; Male; mRNA; Muscle Relaxation - drug effects; Muscle, Smooth, Vascular - drug effects; Myocardium - metabolism; Oligonucleotides - chemical synthesis; Polymerase Chain Reaction; rat aorta rings; Rats; Rats, Wistar; Receptors, Adrenergic, beta-1 - metabolism; RNA - metabolism; RNA, Messenger - metabolism; Tissues, organs and organisms biophysics; Transcription, Genetic - drug effects; Heart; Rat; Rodentia; Smooth muscle; Biosynthesis; Gene expression; Endotoxin; Stress; Muscular relaxation; Vertebrata; Mammalia; Ex vivo; Blood vessel; Animal; Nitric oxide; Bradykinin; Thermal shock; Aorta; Circulatory system; Biological receptor

1 This ex vivo study was performed to characterize B1 receptor induction in rats submitted to heat stress. Changes in aortic isometric tension were recorded after a 90 min in vitro incubation with [des‐Arg9]‐bradykinin. B1 receptor mRNA were detected in aorta and heart using RT–PCR technique. 2 Aort...