Resting B-cells, EBV-infected B-blasts and established lymphoblastoid cell lines differ in their Rb, p53 and EBNA-5 expression patterns

• Published in: Oncogene Vol. 10; no. 9; p. 1869
• Main Authors: Szekely, L, Pokrovskaja, K, Jiang, W Q, Selivanova, G, Löwbeer, M, Ringertz, N, Wiman, K G, Klein, G
• Format: Journal Article
• Language: English
• Published: England 04.05.1995
• Subjects: Antigens, Viral - metabolism; B-Lymphocytes - cytology; B-Lymphocytes - metabolism; Cell Line; DNA-Binding Proteins - metabolism; Epstein-Barr Virus Nuclear Antigens; Fluorescent Antibody Technique; Herpesviridae Infections - metabolism; Herpesvirus 4, Human; Humans; Proliferating Cell Nuclear Antigen - metabolism; Receptors, IgE - metabolism; Retinoblastoma Protein - metabolism; Time Factors; Tumor Suppressor Protein p53 - metabolism; Tumor Virus Infections - metabolism

Using immunofluorescence technique we have analysed the Rb, p53, EBNA-2 and EBNA-5 expression pattern in EBV infected human B-cells and established lymphoblastoid cell lines (LCL-s). Resting B-cells showed only a faint Rb and no p53 immunostaining. The expression of both Rb and p53 increased after E...