Exogenous transforming growth factor‐β1 enhances smooth muscle differentiation in embryonic mouse jejunal explants

• Published in: Journal of tissue engineering and regenerative medicine Vol. 12; no. 1; pp. 252 - 264
• Main Authors: Coletta, Riccardo, Roberts, Neil A., Randles, Michael J., Morabito, Antonino, Woolf, Adrian S.
• Format: Journal Article
• Language: English
• Published: England Hindawi Limited 01.01.2018; John Wiley and Sons Inc
• Subjects: Amniotic fluid; Animals; Cell culture; Cell Differentiation - drug effects; Cell Proliferation - drug effects; culture; Culture media; Differentiation; Digestive system; DNA microarrays; Embryo, Mammalian - drug effects; Explants; Gastrointestinal tract; Gestation; Growth factors; Humans; Intestine; Jejunum - embryology; longitudinal; mesenchyme; Mice; microarray; Muscles; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - drug effects; organ; Organ culture; Organs; Peristalsis; Proteins; Receptors; Receptors, Transforming Growth Factor beta - metabolism; Regenerative medicine; RNA, Messenger - genetics; RNA, Messenger - metabolism; Smooth muscle; Tissue engineering; Transcriptome - genetics; Transforming Growth Factor beta1 - pharmacology; Transforming growth factor-b1; organ; mesenchyme; microarray; intestine; longitudinal; culture

An ex vivo experimental strategy that replicates in vivo intestinal development would in theory provide an accessible setting with which to study normal and dysmorphic gut biology. The current authors recently described a system in which mouse embryonic jejunal segments were explanted onto semiperme...