Fatty acids and gene expression responses to bioactive compounds in Atlantic salmon (Salmo salar L.) hepatocytes

• Published in: Neuro-endocrinology letters Vol. 32 Suppl 2; p. 41
• Main Authors: Schiller Vestergren, Anna Lotta, Trattner, Sofia, Mráz, Jan, Ruyter, Bente, Pickova, Jana
• Format: Journal Article
• Language: English
• Published: Sweden 2011
• Subjects: Animals; Cell Separation; Cell Survival - drug effects; Culture Media; Dioxoles - pharmacology; DNA, Complementary - biosynthesis; DNA, Complementary - genetics; Fatty Acids - metabolism; Gene Expression - drug effects; Genistein - pharmacology; Hepatocytes - drug effects; Hepatocytes - metabolism; In Vitro Techniques; Indicators and Reagents; Lignans - pharmacology; Lipid Metabolism - drug effects; Lipid Metabolism - genetics; Peroxisome Proliferator-Activated Receptors - metabolism; Real-Time Polymerase Chain Reaction; Salmo salar - genetics; Salmo salar - metabolism; Thioctic Acid - pharmacology
• ISSN: 0172-780X

Effects of bioactive compounds on expression of genes involved in lipid metabolism and fatty acid composition were investigated in Atlantic salmon (Salmo salar L.) primary hepatocytes. Five treatments were investigated: I) genistein 0.005 mM, II) genistein 0.025 mM, III) lipoic acid 0.2 mM, IV) sesamin/episesamin 0.05 mM, V) sesamin 0.05 mM and compared to controls. The relative expression of genes involved in lipid homeostasis was analysed after 12h and 48h. Incubation with lipoic acid, sesamin and episesamin/sesamin for 48h had significant effect on all analysed genes involved in lipid uptake, β-oxidation, elongation and desaturation, some effects were detected on the expression of peroxisome prolifertor-activated receptor (PPARs). Also effects on the fatty acid composition were found. The strongest effect of bioactive copounds on hepatocyte gene expression was detected after 48 hours.