Novel and immortalization-based protocols for the generation of neural CNS stem cell lines for gene therapy approaches

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 438; p. 319
• Main Authors: Conti, Luciano, Cattaneo, Elena
• Format: Journal Article
• Language: English
• Published: United States 2008
• Subjects: Animals; Cell Culture Techniques - methods; Cell Line, Transformed; Cell Proliferation; Central Nervous System - cytology; Genetic Therapy - methods; Humans; Mice; Neurons - cytology; Retroviridae; Stem Cells - cytology; Transfection

Transplantation of neural cells engineered to produce growth factors or molecules with antitumor effects have the potential of grafted cells to be used as vectors for protein delivery in animal models of diseases. In this context, neural stem cells (NSCs), since their identification, have been consi...