Bordetella pertussis-specific Th1/Th2 cells generated following respiratory infection or immunization with an acellular vaccine: comparison of the T cell cytokine profiles in infants and mice

• Published in: Developments in biological standardization Vol. 89; p. 297
• Main Authors: Ryan, M, Gothefors, L, Storsaeter, J, Mills, K H
• Format: Journal Article
• Language: English
• Published: Switzerland 1997
• Subjects: Adult; Animals; Antibodies, Bacterial - biosynthesis; Bordetella pertussis - immunology; Cytokines - biosynthesis; Cytokines - immunology; Cytokines - metabolism; Diphtheria-Tetanus-acellular Pertussis Vaccines; Diphtheria-Tetanus-Pertussis Vaccine - immunology; Humans; Immunity, Cellular; Infant; Mice; Th1 Cells - immunology; Th1 Cells - microbiology; Th2 Cells - immunology; Th2 Cells - microbiology; Vaccination; Whooping Cough - immunology; Whooping Cough - metabolism; Whooping Cough - prevention & control
• ISSN: 0301-5149

In an investigation of cell-mediated immunity against Bordetella pertussis, we found that B. pertussis infection in infants and in mice was associated with the induction of antigen-specific T cells that secrete IFN-g and IL-2, but not IL-4 or IL-5. This cytokine profile is characteristic of Th1 cells that mediate cellular immune responses against a range of intracellular pathogens. An examination of cytokine production following immunization with a three-component acellular vaccine, comprising inactive PT, FHA and pertactin adsorbed to alum, demonstrated that spleen cells from vaccinated mice produced high levels of IL-5, but no detectable IFN-g and low levels of IL-2. In contrast, peripheral blood mononuclear cells from vaccinated infants produced IL-2, IL-5 and IFN-g. These findings highlight significant differences in the immune responses generated by vaccination and natural infection with B. pertussis and demonstrate that the T-cell response induced with an acellular vaccine, although dominated by type 2 cytokines in mice, is more heterogeneous in infants with a Th0 or mixed Th1/Th2 cytokine profile.