Immunhistochemical Analysis for Expression of Calpain 1, Calpain 2 and Calpastatin in Endometrial Cancer
Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor is calpastatin. When calcium is present, calpastatin and calpain attach to each other, inhibiting the protease. The calpain system plays an im...
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| Published in | Anticancer research Vol. 30; no. 7; pp. 2837 - 2843 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Attiki
International Institute of Anticancer Research
01.07.2010
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| Abstract | Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor is calpastatin. When calcium is present, calpastatin and calpain attach to each other, inhibiting the protease. The calpain system plays an important role in many processes including apoptosis, necrosis, ischaemia and exocytosis. The role of calpains in pathogenesis or further tumour progression has been proved in related studies. This study focused on the expression of the enzymes calpain 1, calpain 2 and the inhibitor calpastatin in normal and malignant endometrial tissue.
Immunohistochemical stainings were performed on paraffin slices and staining intensity, percentage of positive cells and international ratio score were evaluated.
The endometrial carcinoma showed a higher expression of calpastatin than benign endometrial tissue. |
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| AbstractList | BACKGROUNDCalpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor is calpastatin. When calcium is present, calpastatin and calpain attach to each other, inhibiting the protease. The calpain system plays an important role in many processes including apoptosis, necrosis, ischaemia and exocytosis. The role of calpains in pathogenesis or further tumour progression has been proved in related studies. This study focused on the expression of the enzymes calpain 1, calpain 2 and the inhibitor calpastatin in normal and malignant endometrial tissue.MATERIALS AND METHODSImmunohistochemical stainings were performed on paraffin slices and staining intensity, percentage of positive cells and international ratio score were evaluated.RESULTS AND CONCLUSIONThe endometrial carcinoma showed a higher expression of calpastatin than benign endometrial tissue. Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor is calpastatin. When calcium is present, calpastatin and calpain attach to each other, inhibiting the protease. The calpain system plays an important role in many processes including apoptosis, necrosis, ischaemia and exocytosis. The role of calpains in pathogenesis or further tumour progression has been proved in related studies. This study focused on the expression of the enzymes calpain 1, calpain 2 and the inhibitor calpastatin in normal and malignant endometrial tissue. Immunohistochemical stainings were performed on paraffin slices and staining intensity, percentage of positive cells and international ratio score were evaluated. The endometrial carcinoma showed a higher expression of calpastatin than benign endometrial tissue. Background: Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor is calpastatin. When calcium is present, calpastatin and calpain attach to each other, inhibiting the protease. The calpain system plays an important role in many processes including apoptosis, necrosis, ischaemia and exocytosis. The role of calpains in pathogenesis or further tumour progression has been proved in related studies. This study focused on the expression of the enzymes calpain 1, calpain 2 and the inhibitor calpastatin in normal and malignant endometrial tissue. Materials and Methods: Immunohistochemical stainings were performed on paraffin slices and staining intensity, percentage of positive cells and international ratio score were evaluated. Results and conclusion: The endometrial carcinoma showed a higher expression of calpastatin than benign endometrial tissue. |
| Author | MAASS, Nicolai DOHMEN, Birgit GEORG, Thomas SALEHIN, Darius FRIEDRICH, Michael FROMBERG, Iris BAUERSCHLAG, Dirk HAUGK, Christina BOHLE, Rainer Maria |
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| Keywords | Endometrium cancer Calpastatin Enzyme Cysteine endopeptidases Calpain Malignant tumor Female genital diseases Peptidases Cancerology Uterine diseases endometrial cancer Hydrolases Cancer |
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| Snippet | Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor... BACKGROUNDCalpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific... Background: Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific... |
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| SubjectTerms | Biological and medical sciences Calcium-Binding Proteins - biosynthesis Calpain - biosynthesis Endometrial Neoplasms - enzymology Endometrial Neoplasms - metabolism Endometrial Neoplasms - pathology Female Female genital diseases Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Medical sciences Middle Aged Neoplasm Staging Tumors |
| Title | Immunhistochemical Analysis for Expression of Calpain 1, Calpain 2 and Calpastatin in Endometrial Cancer |
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