Antiviral and antiparasite properties of an L-amino acid oxidase from the Snake Bothrops jararaca : Cloning and identification of a complete cDNA sequence

• Published in: Biochemical pharmacology Vol. 76; no. 2; pp. 279 - 288
• Main Authors: SANT'ANA, Carolina D, MENALDO, Danilo L, SOARES, Andreimar M, COSTA, Tassia R, GODOY, Harryson, MULLER, Vanessa D. M, AQUINO, Victor H, ALBUQUERQUE, Sérgio, SAMPAIO, Suely V, MONTEIRO, Marta C, STABELI, Rodrigo G
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Science 15.07.2008
• Subjects: Aedes; Amino Acid Sequence; Animals; Antiprotozoal Agents - pharmacology; Antiviral Agents - pharmacology; Biological and medical sciences; Bothrops; Bothrops jararaca; Cell Line; Cell Survival - drug effects; Cloning, Molecular; Crotalid Venoms - chemistry; Dengue virus; Dengue Virus - drug effects; DNA, Complementary - genetics; L-Amino Acid Oxidase - genetics; L-Amino Acid Oxidase - pharmacology; Leishmania - drug effects; Medical sciences; Molecular Sequence Data; Pharmacology. Drug treatments; Trypanosoma cruzi - drug effects; cDNA sequence; Snake venom; Nucleotide sequence; Enzyme; Bothrops jararaca; L-Amino-acid oxidase; Identification; Ophidia; Vertebrata; Venom; Parasiticide; Antiviral; Reptilia; Oxidoreductases; L-Amino acid oxidase
• ISSN: 0006-2952; 1873-2968
• DOI: 10.1016/j.bcp.2008.05.003

L-Amino acid oxidases (LAAOs, EC 1.4.3.2) are flavoenzymes that catalyze the stereospecific oxidative deamination of an L-amino acid substrate to the corresponding alpha-ketoacid with hydrogen peroxide and ammonia production. The present work describes the first report on the antiviral (Dengue virus) and antiprotozoal (trypanocidal and leishmanicide) activities of a Bothrops jararaca L-amino acid oxidase (BjarLAAO-I) and identify its cDNA sequence. Antiparasite effects were inhibited by catalase, suggesting that they are mediated by H2O2 production. Cells infected with DENV-3 virus previously treated with BjarLAAO-I, showed a decrease in viral titer (13-83-fold) when compared with cells infected with untreated viruses. Untreated and treated promastigotes (T. cruzi and L. amazonensis) were observed by transmission electron microscopy with different degrees of damage. Its complete cDNA sequence, with 1452 bp, encoded an open reading frame of 484 amino acid residues with a theoretical molecular weight and pI of 54,771.8 and 5.7, respectively. The cDNA-deduced amino acid sequence of BjarLAAO shows high identity to LAAOs from other snake venoms. Further investigations will be focused on the related molecular and functional correlation of these enzymes. Such a study should provide valuable information for the therapeutic development of new generations of microbicidal drugs.