Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: Purification and biochemical characterization

• Published in: Folia microbiologica Vol. 48; no. 5; pp. 627 - 632
• Main Authors: GUIMARAES, L. H. S, TERENZI, H. F, JORGE, J. A, LEONE, F. A, POLIZELI, M. L. T. M
• Format: Journal Article
• Language: English
• Published: Praha Academia 2003
• Subjects: Alkaline Phosphatase - chemistry; Alkaline Phosphatase - isolation & purification; Alkaline Phosphatase - metabolism; Aspergillus - enzymology; Aspergillus caespitosus; Biological and medical sciences; Carbohydrates; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Glycoproteins - chemistry; Glycoproteins - isolation & purification; Glycoproteins - metabolism; Hydrogen-Ion Concentration; Kinetics; Microbiology; Miscellaneous; Molecular Weight; Mycology; Substrate Specificity; Temperature; Fungi; Alkaline phosphatase; Aspergillus caespitosus; Purification; Microbiology; Enzyme; Phosphoric monoester hydrolases; Hydrolases; Esterases; Fungi Imperfecti; Thallophyta

Among 30 species of filamentous fungi isolated from Brazilian soil, Aspergillus caespitosus produced and secreted the highest levels of alkaline phosphatase in culture medium supplemented with xylan. The extracellular alkaline phosphatase was purified by DEAE-cellulose and concanavalin A-sepharose c...