COMPARISON OF POUZOLZIA INDICA METHANOLIC EXTRACT AND VIRKON® AGAINST CYSTS OF ACANTHAMOEBA SPP

The present study was conducted to investigate the morphological and structural changes of Acanthamoeba cysts after being treated with various concentrations of Pouzolzia indica methanolic extract fraction 3 (methanol eluted) and Virkon solution. Changes in the Acanthamoeba cysts were detected by li...

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Published inSoutheast Asian journal of tropical medicine and public health Vol. 41; no. 4; pp. 776 - 784
Main Authors ROONGRUANGCHAI, Kosol, KUMMALUE, Tanawan, SOOKKUA, Tichaporn, ROONGRUANGCHAI, Jantima
Format Journal Article
LanguageEnglish
Published Bangkok SEAMO, Regional Tropical Medicine and Public Health Network 01.07.2010
Central Coordinating Board, SEAMEO-TROPMED Project
Subjects
Acanthamoeba - drug effects
Antiprotozoal Agents - pharmacology
Biological and medical sciences
Cells, Cultured
General aspects
Medical sciences
Microscopy, Electron, Scanning
Peroxides - pharmacology
Plant Extracts - pharmacology
Sulfuric Acids - pharmacology
Urticaceae
Protozoa
Lobosea
Acanthamoeba
Cyst
Tropical medicine
Benign neoplasm
Comparative study
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Summary:The present study was conducted to investigate the morphological and structural changes of Acanthamoeba cysts after being treated with various concentrations of Pouzolzia indica methanolic extract fraction 3 (methanol eluted) and Virkon solution. Changes in the Acanthamoeba cysts were detected by light microscopy, scanning electron microscopy and transmission electron microscopy. The results show Acanthamoeba cysts were killed by Pouzolzia indica methanolic extract fraction 3 at a concentration of 1:8 and by Virkon solution at a concentration of 0.25%, with a minimal cysticidal concentration (MCC) by 24 hours. Both agents caused similar structural damage to Acanthamoeba cysts in the same sequence. Step by step structural alterations occurred within the cyst. First, the cyst shrank, collapsed and had clumping of cytoplasmic stuctures inside the cyst walls. Second, the cysts began to bulge, swell, have a decrease in wrinkles in the cyst walls and spill the cytoplasmic contents into the environment. Finally, the cyst walls broke into small pieces. This study may be beneficial to compare with future studies of pharmaceutical agents against Acanthamoeba keratitis.
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ISSN:0125-1562