Stromal cells maintain the radioprotective capacity of CFU-S during retroviral infection

• Published in: Gene therapy Vol. 3; no. 9; p. 761
• Main Authors: Goncalves, F, Dubart, A, Lacout, C, Vainchenker, W, Duménil, D
• Format: Journal Article
• Language: English
• Published: England 01.09.1996
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Cell Adhesion; Cell Line; Coculture Techniques; Gene Transfer Techniques; Genetic Vectors - physiology; Gentamicins - pharmacology; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells - cytology; Kanamycin Kinase; Male; Mice; Mice, Inbred CBA; Phosphotransferases (Alcohol Group Acceptor) - genetics; Radiation Tolerance; Retroviridae - physiology; Spleen - cytology; Stromal Cells - physiology; Virus Integration

Retroviral vectors provide an efficient means to introduce genes into hematopoietic stem cells. In order to develop retroviral infection protocols which preserve the radioprotective capacity of CFU-S, we designed a clonal hematopoietic reconstitution assay. In this assay, single CFU-S-derived derive...